KUKU

KU ScholarWorks

  • myKU
  • Email
  • Enroll & Pay
  • KU Directory
    • Login
    View Item 
    •   KU ScholarWorks
    • Dissertations and Theses
    • Dissertations
    • View Item
    •   KU ScholarWorks
    • Dissertations and Theses
    • Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Improvement of the chemical and physical stability of the EC1 domain of E-cadherin by blocking its disulfide-mediated dimerization

    Thumbnail
    View/Open
    umi-ku-2368_1.pdf (1.216Mb)
    Issue Date
    2008-01-30
    Author
    Trivedi, Maulik
    Publisher
    University of Kansas
    Format
    194 pages
    Type
    Dissertation
    Degree Level
    PH.D.
    Discipline
    Pharmaceutical Chemistry
    Rights
    This item is protected by copyright and unless otherwise specified the copyright of this thesis/dissertation is held by the author.
    Metadata
    Show full item record
    Abstract
    The EC1 protein has an important role in the E-cadherin mediated cell-cell adhesion in epithelial and endothelial tissues. It may be used as modulator of cellular adhesion to improve paracellular delivery of macromolecules. EC1 undergoes oxidation of its Cys residue to form disulfide-linked covalent dimers. These dimers associate to form physical oligomers. The dimerization and oligomerization also lead to hydrolysis of the Asp93-Pro94 peptide bond and precipitation. To be able to study the cell adhesion-modulating activity of EC1, it is important that we block its disulfide-dimerization and subsequent oligomerization. The strategies used to block the dimerization were addition of dithiothreitol to the EC1 solution, modification of the Cys thiol with iodoacetamide and polyethylene glycol. These derivatives were studied for their stability using HPLC, CD and fluorescence spectroscopy. All strategies applied showed improvement in the stability of EC1. The PEGylated EC1 showed the best stability.
    URI
    http://hdl.handle.net/1808/4099
    Collections
    • Dissertations [4473]
    • Pharmaceutical Chemistry Dissertations and Theses [141]

    Items in KU ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.


    We want to hear from you! Please share your stories about how Open Access to this item benefits YOU.


    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

    Browse

    All of KU ScholarWorksCommunities & CollectionsThis Collection

    My Account

    LoginRegister

    Statistics

    View Usage Statistics

    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

    The University of Kansas
      Contact KU ScholarWorks
    Lawrence, KS | Maps
     
    • Academics
    • Admission
    • Alumni
    • Athletics
    • Campuses
    • Giving
    • Jobs

    The University of Kansas prohibits discrimination on the basis of race, color, ethnicity, religion, sex, national origin, age, ancestry, disability, status as a veteran, sexual orientation, marital status, parental status, gender identity, gender expression and genetic information in the University’s programs and activities. The following person has been designated to handle inquiries regarding the non-discrimination policies: Director of the Office of Institutional Opportunity and Access, IOA@ku.edu, 1246 W. Campus Road, Room 153A, Lawrence, KS, 66045, (785)864-6414, 711 TTY.

     Contact KU
    Lawrence, KS | Maps