Separation and Detection of Tyrosine and Phenylalanine-derived Oxidative Stress Biomarkers Using Microchip Electrophoresis with Electrochemical Detection

Weerasekara, Dhanushka B.; Lunte, Susan M.

dc.contributor.author	Weerasekara, Dhanushka B.
dc.contributor.author	Lunte, Susan M.
dc.date.accessioned	2023-06-13T15:54:07Z
dc.date.available	2023-06-13T15:54:07Z
dc.date.issued	2021-12-31
dc.identifier.citation	Weerasekara, D. B., & Lunte, S. M. (2022). Separation and Detection of Tyrosine and Phenylalanine-derived Oxidative Stress Biomarkers Using Microchip Electrophoresis with Electrochemical Detection. Electroanalysis, 34(12), 1913–1927. https://doi.org/10.1002/elan.202100580	en_US
dc.identifier.uri	https://hdl.handle.net/1808/34350
dc.description	This is the peer reviewed version of the following article: D. B. Weerasekara, S. M. Lunte, Electroanalysis 2022, 34, 1913, which has been published in final form at https://doi.org/10.1002/elan.202100580. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.	en_US
dc.description.abstract	A method for the determination of selected aromatic amino acid biomarkers of oxidative stress using microchip electrophoresis with electrochemical detection is described. The separation of the major reaction products of phenylalanine and tyrosine with reactive nitrogen and oxygen species was accomplished using ligand exchange micellar electrokinetic chromatography with a PDMS/glass hybrid chip. Electrochemical detection was achieved using a pyrolyzed photoresist film working electrode. The system was evaluated for the analysis of the products of the Fenton reaction with tyrosine and phenylalanine, and the reaction of peroxynitrite with tyrosine.	en_US
dc.publisher	Wiley	en_US
dc.rights	© 2022 Wiley-VCH GmbH	en_US
dc.subject	Reactive nitrogen and oxygen species	en_US
dc.subject	Microchip electrophoresis with electrochemical detection	en_US
dc.subject	Hydroxyl radical	en_US
dc.subject	Peroxynitrite	en_US
dc.subject	Tyrosine	en_US
dc.title	Separation and Detection of Tyrosine and Phenylalanine-derived Oxidative Stress Biomarkers Using Microchip Electrophoresis with Electrochemical Detection	en_US
dc.type	Article	en_US
dc.identifier.doi	10.1002/elan.202100580	en_US
dc.identifier.orcid	https://orcid.org/0000-0002-1195-0314	en_US
kusw.oaversion	Scholarly/refereed, author accepted manuscript	en_US
kusw.oapolicy	This item meets KU Open Access policy criteria.	en_US
dc.identifier.pmid	PMC10202003	en_US
dc.rights.accessrights	openAccess	en_US

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