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Cell activation-based screening of natively paired human T cell receptor repertoires
dc.contributor.author | Fahad, Ahmed S. | |
dc.contributor.author | Chung, Cheng Yu | |
dc.contributor.author | López Acevedo, Sheila N. | |
dc.contributor.author | Boyle, Nicoleen | |
dc.contributor.author | Madan, Bharat | |
dc.contributor.author | Gutiérrez-González, Matías F. | |
dc.contributor.author | Matus-Nicodemos, Rodrigo | |
dc.contributor.author | Laflin, Amy D. | |
dc.contributor.author | Ladi, Rukmini R. | |
dc.contributor.author | Zhou, John | |
dc.contributor.author | Wolfe, Jacy | |
dc.contributor.author | Llewellyn-Lacey, Sian | |
dc.contributor.author | Koup, Richard A. | |
dc.contributor.author | Douek, Daniel C. | |
dc.contributor.author | Balfour, Henry H., Jr. | |
dc.contributor.author | Price, David A. | |
dc.contributor.author | DeKosky, Brandon J. | |
dc.date.accessioned | 2023-06-12T20:08:47Z | |
dc.date.available | 2023-06-12T20:08:47Z | |
dc.date.issued | 2023-05-17 | |
dc.identifier.citation | Fahad, A.S., Chung, C.Y., López Acevedo, S.N. et al. Cell activation-based screening of natively paired human T cell receptor repertoires. Sci Rep 13, 8011 (2023). https://doi.org/10.1038/s41598-023-31858-4 | en_US |
dc.identifier.uri | https://hdl.handle.net/1808/34332 | |
dc.description.abstract | Adoptive immune therapies based on the transfer of antigen-specific T cells have been used successfully to treat various cancers and viral infections, but improved techniques are needed to identify optimally protective human T cell receptors (TCRs). Here we present a high-throughput approach to the identification of natively paired human TCRα and TCRβ (TCRα:β) genes encoding heterodimeric TCRs that recognize specific peptide antigens bound to major histocompatibility complex molecules (pMHCs). We first captured and cloned TCRα:β genes from individual cells, ensuring fidelity using a suppression PCR. We then screened TCRα:β libraries expressed in an immortalized cell line using peptide-pulsed antigen-presenting cells and sequenced activated clones to identify the cognate TCRs. Our results validated an experimental pipeline that allows large-scale repertoire datasets to be annotated with functional specificity information, facilitating the discovery of therapeutically relevant TCRs. | en_US |
dc.publisher | Nature Research | en_US |
dc.rights | © The Author(s) 2023. This article is licensed under a Creative Commons Attribution 4.0 International License. | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en_US |
dc.subject | Biotechnology | en_US |
dc.subject | Molecular biology | en_US |
dc.title | Cell activation-based screening of natively paired human T cell receptor repertoires | en_US |
dc.type | Article | en_US |
kusw.kuauthor | Fahad, Ahmed S. | |
kusw.kuauthor | Chung, Cheng Yu | |
kusw.kuauthor | López Acevedo, Sheila N. | |
kusw.kuauthor | Boyle, Nicoleen | |
kusw.kuauthor | Madan, Bharat | |
kusw.kuauthor | Gutiérrez-González, Matías F. | |
kusw.kuauthor | Laflin, Amy D. | |
kusw.kuauthor | Ladi, Rukmini R. | |
kusw.kuauthor | Zhou, John | |
kusw.kuauthor | Wolfe, Jacy | |
kusw.kuauthor | DeKosky, Brandon J. | |
kusw.kudepartment | Pharmaceutical Chemistry | en_US |
kusw.kudepartment | Chemical Engineering | en_US |
dc.identifier.doi | 10.1038/s41598-023-31858-4 | en_US |
kusw.oaversion | Scholarly/refereed, publisher version | en_US |
kusw.oapolicy | This item meets KU Open Access policy criteria. | en_US |
dc.identifier.pmid | PMC10192375 | en_US |
dc.rights.accessrights | openAccess | en_US |