dc.contributor.author | Jarosova, Romana | |
dc.contributor.author | Woolfolk, Sarah K. | |
dc.contributor.author | Martinez-Rivera, Noraida | |
dc.contributor.author | Jaeschke, Mathew W. | |
dc.contributor.author | Rosa-Molinar, Eduardo | |
dc.contributor.author | Tamerler, Candan | |
dc.contributor.author | Johnson, Michael A. | |
dc.date.accessioned | 2023-04-11T19:14:07Z | |
dc.date.available | 2023-04-11T19:14:07Z | |
dc.date.issued | 2023-02-28 | |
dc.identifier.citation | Jarosova, R.; Woolfolk, S.K.; Martinez-Rivera, N.; Jaeschke, M.W.; Rosa-Molinar, E.; Tamerler, C.; Johnson, M.A. Spatiotemporal Imaging of Zinc Ions in Zebrafish Live Brain Tissue Enabled by Fluorescent Bionanoprobes. Molecules 2023, 28, 2260. https://doi.org/10.3390/molecules28052260 | en_US |
dc.identifier.uri | https://hdl.handle.net/1808/34095 | |
dc.description.abstract | The zebrafish is a powerful model organism to study the mechanisms governing transition metal ions within whole brain tissue. Zinc is one of the most abundant metal ions in the brain, playing a critical pathophysiological role in neurodegenerative diseases. The homeostasis of free, ionic zinc (Zn2+) is a key intersection point in many of these diseases, including Alzheimer’s disease and Parkinson’s disease. A Zn2+ imbalance can eventuate several disturbances that may lead to the development of neurodegenerative changes. Therefore, compact, reliable approaches that allow the optical detection of Zn2+ across the whole brain would contribute to our current understanding of the mechanisms that underlie neurological disease pathology. We developed an engineered fluorescence protein-based nanoprobe that can spatially and temporally resolve Zn2+ in living zebrafish brain tissue. The self-assembled engineered fluorescence protein on gold nanoparticles was shown to be confined to defined locations within the brain tissue, enabling site specific studies, compared to fluorescent protein-based molecular tools, which diffuse throughout the brain tissue. Two-photon excitation microscopy confirmed the physical and photometrical stability of these nanoprobes in living zebrafish (Danio rerio) brain tissue, while the addition of Zn2+ quenched the nanoprobe fluorescence. Combining orthogonal sensing methods with our engineered nanoprobes will enable the study of imbalances in homeostatic Zn2+ regulation. The proposed bionanoprobe system offers a versatile platform to couple metal ion specific linkers and contribute to the understanding of neurological diseases. | en_US |
dc.publisher | MDPI | en_US |
dc.rights | © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license. | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en_US |
dc.subject | Zinc | en_US |
dc.subject | Gold | en_US |
dc.subject | Nanoparticles | en_US |
dc.subject | Zebrafish | en_US |
dc.subject | Two-photon excitation imaging | en_US |
dc.subject | Fluorescence | en_US |
dc.title | Spatiotemporal Imaging of Zinc Ions in Zebrafish Live Brain Tissue Enabled by Fluorescent Bionanoprobes | en_US |
dc.type | Article | en_US |
kusw.kuauthor | Jarosova, Romana | |
kusw.kuauthor | Woolfolk, Sarah K. | |
kusw.kuauthor | Martinez-Rivera, Noraida | |
kusw.kuauthor | Jaeschke, Mathew W. | |
kusw.kuauthor | Rosa-Molinar, Eduardo | |
kusw.kuauthor | Tamerler, Candan | |
kusw.kuauthor | Johnson, Michael A. | |
kusw.kudepartment | Chemistry | en_US |
kusw.kudepartment | R.N. Adams Institute for Bioanalytical Chemistry | en_US |
kusw.kudepartment | Institute for Bioengineering Research | en_US |
kusw.kudepartment | Bioengineering Program | en_US |
kusw.kudepartment | Microscopy and Analytical Imaging Research Resource Core Laboratory | en_US |
kusw.kudepartment | Mechanical Engineering | en_US |
kusw.kudepartment | Pharmacology & Toxicology | en_US |
dc.identifier.doi | 10.3390/molecules28052260 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0001-6721-4394 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0003-2057-8937 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-1960-2218 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0001-5078-9896 | en_US |
kusw.oaversion | Scholarly/refereed, publisher version | en_US |
kusw.oapolicy | This item meets KU Open Access policy criteria. | en_US |
dc.identifier.pmid | PMC10005619 | en_US |
dc.rights.accessrights | openAccess | en_US |