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Pseudomonas aeruginosa Dps (PA0962) Functions in H2O2 Mediated Oxidative Stress Defense and Exhibits In Vitro DNA Cleaving Activity
dc.contributor.author | Rajapaksha, Nimesha | |
dc.contributor.author | Soldano, Anabel | |
dc.contributor.author | Yao, Huili | |
dc.contributor.author | Donnarumma, Fabrizio | |
dc.contributor.author | Kashipathy, Maithri M. | |
dc.contributor.author | Seibold, Steve | |
dc.contributor.author | Battaile, Kevin P. | |
dc.contributor.author | Lovell, Scott | |
dc.contributor.author | Rivera, Mario | |
dc.date.accessioned | 2023-04-10T18:30:53Z | |
dc.date.available | 2023-04-10T18:30:53Z | |
dc.date.issued | 2023-02-28 | |
dc.identifier.citation | Rajapaksha, N.; Soldano, A.; Yao, H.; Donnarumma, F.; Kashipathy, M.M.; Seibold, S.; Battaile, K.P.; Lovell, S.; Rivera, M. Pseudomonas aeruginosa Dps (PA0962) Functions in H2O2 Mediated Oxidative Stress Defense and Exhibits In Vitro DNA Cleaving Activity. Int. J. Mol. Sci. 2023, 24, 4669. https://doi.org/10.3390/ijms24054669 | en_US |
dc.identifier.uri | https://hdl.handle.net/1808/34085 | |
dc.description.abstract | We report the structural, biochemical, and functional characterization of the product of gene PA0962 from Pseudomonas aeruginosa PAO1. The protein, termed Pa Dps, adopts the Dps subunit fold and oligomerizes into a nearly spherical 12-mer quaternary structure at pH 6.0 or in the presence of divalent cations at neutral pH and above. The 12-Mer Pa Dps contains two di-iron centers at the interface of each subunit dimer, coordinated by conserved His, Glu, and Asp residues. In vitro, the di-iron centers catalyze the oxidation of Fe2+ utilizing H2O2 (not O2) as an oxidant, suggesting Pa Dps functions to aid P. aeruginosa to survive H2O2-mediated oxidative stress. In agreement, a P. aeruginosa Δdps mutant is significantly more susceptible to H2O2 than the parent strain. The Pa Dps structure harbors a novel network of Tyr residues at the interface of each subunit dimer between the two di-iron centers, which captures radicals generated during Fe2+ oxidation at the ferroxidase centers and forms di-tyrosine linkages, thus effectively trapping the radicals within the Dps shell. Surprisingly, incubating Pa Dps and DNA revealed unprecedented DNA cleaving activity that is independent of H2O2 or O2 but requires divalent cations and 12-mer Pa Dps. | en_US |
dc.publisher | MDPI | en_US |
dc.rights | © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license. | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en_US |
dc.subject | Dps | en_US |
dc.subject | Mini ferritin | en_US |
dc.subject | Ferritin | en_US |
dc.subject | Iron metabolism | en_US |
dc.subject | Oxidative stress | en_US |
dc.subject | Peroxide toxicity | en_US |
dc.subject | Exonuclease | en_US |
dc.subject | DNA cleaving activity | en_US |
dc.title | Pseudomonas aeruginosa Dps (PA0962) Functions in H2O2 Mediated Oxidative Stress Defense and Exhibits In Vitro DNA Cleaving Activity | en_US |
dc.type | Article | en_US |
kusw.kuauthor | Kashipathy, Maithri M. | |
kusw.kuauthor | Seibold, Steve | |
kusw.kuauthor | Lovell, Scott | |
kusw.kudepartment | Protein Structure and X-ray Crystallography Laboratory | en_US |
dc.identifier.doi | 10.3390/ijms24054669 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0003-0833-3259 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-3215-4472 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-5692-5497 | en_US |
kusw.oaversion | Scholarly/refereed, publisher version | en_US |
kusw.oapolicy | This item meets KU Open Access policy criteria. | en_US |
dc.identifier.pmid | PMC10002758 | en_US |
dc.rights.accessrights | openAccess | en_US |