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Mapping monoclonal anti-SARS-CoV-2 antibody repertoires against diverse coronavirus antigens
dc.contributor.author | de Souza, Matheus Oliveira | |
dc.contributor.author | Madan, Bharat | |
dc.contributor.author | Teng, I-Ting | |
dc.contributor.author | Huang, Aric | |
dc.contributor.author | Liu, Lihong | |
dc.contributor.author | Fahad, Ahmed S. | |
dc.contributor.author | Lopez Acevedo, Sheila N. | |
dc.contributor.author | Pan, Xiaoli | |
dc.contributor.author | Sastry, Mallika | |
dc.contributor.author | Gutierrez-Gonzalez, Matias | |
dc.contributor.author | Yin, Michael T. | |
dc.contributor.author | Zhou, Tongqing | |
dc.contributor.author | Ho, David D. | |
dc.contributor.author | Kwong, Peter D. | |
dc.contributor.author | DeKosky, Brandon J. | |
dc.date.accessioned | 2023-02-28T15:20:59Z | |
dc.date.available | 2023-02-28T15:20:59Z | |
dc.date.issued | 2022-09-02 | |
dc.identifier.citation | de Souza MO, Madan B, Teng I-T, Huang A, Liu L, Fahad AS, Lopez Acevedo SN, Pan X, Sastry M, Gutierrez-Gonzalez M, Yin MT, Zhou T, Ho DD, Kwong PD and DeKosky BJ (2022) Mapping monoclonal anti-SARS-CoV-2 antibody repertoires against diverse coronavirus antigens. Front. Immunol. 13:977064. doi: 10.3389/fimmu.2022.977064 | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/33961 | |
dc.description.abstract | Variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have emerged continuously, challenging the effectiveness of vaccines, diagnostics, and treatments. Moreover, the possibility of the appearance of a new betacoronavirus with high transmissibility and high fatality is reason for concern. In this study, we used a natively paired yeast display technology, combined with next-generation sequencing (NGS) and massive bioinformatic analysis to perform a comprehensive study of subdomain specificity of natural human antibodies from two convalescent donors. Using this screening technology, we mapped the cross-reactive responses of antibodies generated by the two donors against SARS-CoV-2 variants and other betacoronaviruses. We tested the neutralization potency of a set of the cross-reactive antibodies generated in this study and observed that most of the antibodies produced by these patients were non-neutralizing. We performed a comparison of the specific and non-specific antibodies by somatic hypermutation in a repertoire-scale for the two individuals and observed that the degree of somatic hypermutation was unique for each patient. The data from this study provide functional insights into cross-reactive antibodies that can assist in the development of strategies against emerging SARS-CoV-2 variants and divergent betacoronaviruses. | en_US |
dc.publisher | Frontiers Media | en_US |
dc.rights | © 2022 de Souza, Madan, Teng, Huang, Liu, Fahad, Lopez Acevedo, Pan, Sastry, Gutierrez-Gonzalez, Yin, Zhou, Ho, Kwong and DeKosky. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en_US |
dc.subject | SARS-CoV-2 | en_US |
dc.subject | Yeast display | en_US |
dc.subject | Betacoronaviruses | en_US |
dc.subject | SARS-CoV-2 variants | en_US |
dc.subject | Cross-reactive antibodies | en_US |
dc.title | Mapping monoclonal anti-SARS-CoV-2 antibody repertoires against diverse coronavirus antigens | en_US |
dc.type | Article | en_US |
kusw.kuauthor | de Souza, Matheus Oliveira | |
kusw.kuauthor | Madan, Bharat | |
kusw.kuauthor | Huang, Aric | |
kusw.kuauthor | Fahad, Ahmed S. | |
kusw.kuauthor | Lopez Acevedo, Sheila N. | |
kusw.kuauthor | Pan, Xiaoli | |
kusw.kuauthor | DeKosky, Brandon J. | |
kusw.kudepartment | Pharmaceutical Chemistry | en_US |
kusw.kudepartment | Chemical Engineering | en_US |
dc.identifier.doi | 10.3389/fimmu.2022.977064 | en_US |
kusw.oaversion | Scholarly/refereed, publisher version | en_US |
kusw.oapolicy | This item meets KU Open Access policy criteria. | en_US |
dc.identifier.pmid | PMC9478573 | en_US |
dc.rights.accessrights | openAccess | en_US |
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Except where otherwise noted, this item's license is described as: © 2022 de Souza, Madan, Teng, Huang, Liu, Fahad, Lopez Acevedo, Pan, Sastry, Gutierrez-Gonzalez, Yin, Zhou, Ho, Kwong and DeKosky. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).