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dc.contributor.authorde Souza, Matheus Oliveira
dc.contributor.authorMadan, Bharat
dc.contributor.authorTeng, I-Ting
dc.contributor.authorHuang, Aric
dc.contributor.authorLiu, Lihong
dc.contributor.authorFahad, Ahmed S.
dc.contributor.authorLopez Acevedo, Sheila N.
dc.contributor.authorPan, Xiaoli
dc.contributor.authorSastry, Mallika
dc.contributor.authorGutierrez-Gonzalez, Matias
dc.contributor.authorYin, Michael T.
dc.contributor.authorZhou, Tongqing
dc.contributor.authorHo, David D.
dc.contributor.authorKwong, Peter D.
dc.contributor.authorDeKosky, Brandon J.
dc.date.accessioned2023-02-28T15:20:59Z
dc.date.available2023-02-28T15:20:59Z
dc.date.issued2022-09-02
dc.identifier.citationde Souza MO, Madan B, Teng I-T, Huang A, Liu L, Fahad AS, Lopez Acevedo SN, Pan X, Sastry M, Gutierrez-Gonzalez M, Yin MT, Zhou T, Ho DD, Kwong PD and DeKosky BJ (2022) Mapping monoclonal anti-SARS-CoV-2 antibody repertoires against diverse coronavirus antigens. Front. Immunol. 13:977064. doi: 10.3389/fimmu.2022.977064en_US
dc.identifier.urihttp://hdl.handle.net/1808/33961
dc.description.abstractVariants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have emerged continuously, challenging the effectiveness of vaccines, diagnostics, and treatments. Moreover, the possibility of the appearance of a new betacoronavirus with high transmissibility and high fatality is reason for concern. In this study, we used a natively paired yeast display technology, combined with next-generation sequencing (NGS) and massive bioinformatic analysis to perform a comprehensive study of subdomain specificity of natural human antibodies from two convalescent donors. Using this screening technology, we mapped the cross-reactive responses of antibodies generated by the two donors against SARS-CoV-2 variants and other betacoronaviruses. We tested the neutralization potency of a set of the cross-reactive antibodies generated in this study and observed that most of the antibodies produced by these patients were non-neutralizing. We performed a comparison of the specific and non-specific antibodies by somatic hypermutation in a repertoire-scale for the two individuals and observed that the degree of somatic hypermutation was unique for each patient. The data from this study provide functional insights into cross-reactive antibodies that can assist in the development of strategies against emerging SARS-CoV-2 variants and divergent betacoronaviruses.en_US
dc.publisherFrontiers Mediaen_US
dc.rights© 2022 de Souza, Madan, Teng, Huang, Liu, Fahad, Lopez Acevedo, Pan, Sastry, Gutierrez-Gonzalez, Yin, Zhou, Ho, Kwong and DeKosky. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).en_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.subjectSARS-CoV-2en_US
dc.subjectYeast displayen_US
dc.subjectBetacoronavirusesen_US
dc.subjectSARS-CoV-2 variantsen_US
dc.subjectCross-reactive antibodiesen_US
dc.titleMapping monoclonal anti-SARS-CoV-2 antibody repertoires against diverse coronavirus antigensen_US
dc.typeArticleen_US
kusw.kuauthorde Souza, Matheus Oliveira
kusw.kuauthorMadan, Bharat
kusw.kuauthorHuang, Aric
kusw.kuauthorFahad, Ahmed S.
kusw.kuauthorLopez Acevedo, Sheila N.
kusw.kuauthorPan, Xiaoli
kusw.kuauthorDeKosky, Brandon J.
kusw.kudepartmentPharmaceutical Chemistryen_US
kusw.kudepartmentChemical Engineeringen_US
dc.identifier.doi10.3389/fimmu.2022.977064en_US
kusw.oaversionScholarly/refereed, publisher versionen_US
kusw.oapolicyThis item meets KU Open Access policy criteria.en_US
dc.identifier.pmidPMC9478573en_US
dc.rights.accessrightsopenAccessen_US


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© 2022 de Souza, Madan, Teng, Huang, Liu, Fahad, Lopez Acevedo, Pan, Sastry, Gutierrez-Gonzalez, Yin, Zhou, Ho, Kwong and DeKosky. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).
Except where otherwise noted, this item's license is described as: © 2022 de Souza, Madan, Teng, Huang, Liu, Fahad, Lopez Acevedo, Pan, Sastry, Gutierrez-Gonzalez, Yin, Zhou, Ho, Kwong and DeKosky. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).