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Microfluidic affinity selection of active SARS-CoV-2 virus particles
dc.contributor.author | Gamage, Sachindra S. T. | |
dc.contributor.author | Pahattuge, Thilanga N. | |
dc.contributor.author | Wijerathne, Harshani | |
dc.contributor.author | Childers, Katie | |
dc.contributor.author | Vaidyanathan, Swarnagowri | |
dc.contributor.author | Athapattu, Uditha S. | |
dc.contributor.author | Zhang, Lulu | |
dc.contributor.author | Zhao, Zheng | |
dc.contributor.author | Hupert, Mateusz L. | |
dc.contributor.author | Muller, Rolf M. | |
dc.contributor.author | Muller-Cohn, Judy | |
dc.contributor.author | Dickerson, Janet | |
dc.contributor.author | Dufek, Dylan | |
dc.contributor.author | Geisbrecht, Brian V. | |
dc.contributor.author | Pathak, Harsh | |
dc.contributor.author | Pessetto, Ziyan | |
dc.contributor.author | Gan, Gregory N. | |
dc.contributor.author | Choi, Junseo | |
dc.contributor.author | Park, Sunggook | |
dc.contributor.author | Godwin, Andrew K. | |
dc.contributor.author | Witek, Malgorzata A. | |
dc.contributor.author | Soper, Steven A. | |
dc.date.accessioned | 2023-02-20T19:40:31Z | |
dc.date.available | 2023-02-20T19:40:31Z | |
dc.date.issued | 2022-09-28 | |
dc.identifier.citation | Gamage, Sachindra S T et al. “Microfluidic affinity selection of active SARS-CoV-2 virus particles.” Science advances vol. 8,39 (2022): eabn9665. doi:10.1126/sciadv.abn9665 | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/33863 | |
dc.description.abstract | We report a microfluidic assay to select active severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral particles (VPs), which were defined as intact particles with an accessible angiotensin-converting enzyme 2 receptor binding domain (RBD) on the spike (S) protein, from clinical samples. Affinity selection of SARS-CoV-2 particles was carried out using injection molded microfluidic chips, which allow for high-scale production to accommodate large-scale screening. The microfluidic contained a surface-bound aptamer directed against the virus’s S protein RBD to affinity select SARS-CoV-2 VPs. Following selection (~94% recovery), the VPs were released from the chip’s surface using a blue light light-emitting diode (89% efficiency). Selected SARS-CoV-2 VP enumeration was carried out using reverse transcription quantitative polymerase chain reaction. The VP selection assay successfully identified healthy donors (clinical specificity = 100%) and 19 of 20 patients with coronavirus disease 2019 (COVID-19) (95% sensitivity). In 15 patients with COVID-19, the presence of active SARS-CoV-2 VPs was found. The chip can be reprogrammed for any VP or exosomes by simply changing the affinity agent. | en_US |
dc.publisher | American Association for the Advancement of Science | en_US |
dc.rights | Copyright © 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S.Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY). | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en_US |
dc.title | Microfluidic affinity selection of active SARS-CoV-2 virus particles | en_US |
dc.type | Article | en_US |
kusw.kuauthor | Gamage, Sachindra S. T. | |
kusw.kuauthor | Pahattuge, Thilanga N. | |
kusw.kuauthor | Wijerathne, Harshani | |
kusw.kuauthor | Childers, Katie | |
kusw.kuauthor | Vaidyanathan, Swarnagowri | |
kusw.kuauthor | Athapattu, Uditha S. | |
kusw.kuauthor | Zhang, Lulu | |
kusw.kuauthor | Zhao, Zheng | |
kusw.kuauthor | Choi, Junseo | |
kusw.kuauthor | Park, Sunggook | |
kusw.kuauthor | Godwin, Andrew K. | |
kusw.kuauthor | Witek, Malgorzata A. | |
kusw.kuauthor | Soper, Steven A. | |
kusw.kudepartment | Chemistry | en_US |
kusw.kudepartment | Center of BioModular Multiscale Systems for Precision Medicine | en_US |
kusw.kudepartment | Bioengineering Program | en_US |
kusw.kudepartment | Mechanical Engineering | en_US |
dc.identifier.doi | 10.1126/sciadv.abn9665 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-7507-3226 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-3961-1664 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-9921-0731 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0003-0397-5543 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0001-7068-1321 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-1876-3976 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-7481-8898 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0003-4599-7812 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-1775-0727 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0003-4034-0520 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-3461-3820 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-3987-9580 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-2802-7235 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-8292-7058 | en_US |
kusw.oaversion | Scholarly/refereed, publisher version | en_US |
kusw.oapolicy | This item meets KU Open Access policy criteria. | en_US |
dc.identifier.pmid | PMC9519043 | en_US |
dc.rights.accessrights | openAccess | en_US |
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Except where otherwise noted, this item's license is described as: Copyright © 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S.Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY).