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Novel hydrogel system eliminates subculturing and improves retention of nonsenescent mesenchymal stem cell populations

dc.contributor.authorHodge, Jacob G.
dc.contributor.authorRobinson, Jennifer L.
dc.contributor.authorMellott, Adam J.
dc.date.accessioned2023-02-09T19:47:00Z
dc.date.available2023-02-09T19:47:00Z
dc.date.issued2022-10-12
dc.identifier.citationHodge, J. G., Robinson, J. L., & Mellott, A. J. (2023). Novel hydrogel system eliminates subculturing and improves retention of nonsenescent mesenchymal stem cell populations. Regenerative medicine, 18(1), 23–36. https://doi.org/10.2217/rme-2022-0140en_US
dc.identifier.urihttp://hdl.handle.net/1808/33764
dc.description.abstractAim: To compare the physiological behavior of mesenchymal stem/stromal cells (MSCs) within an expandable tissue-mimetic 3D system relative to in vitro expansion in a traditional 2D system.

Methods: Adipose-derived MSCs (ASCs) were continuously cultured for 6 weeks on either 2D culture plastic or in a 3D hydrogel system that eliminated subculturing. ASCs were assessed for senescence, ‘stem-like’ MSC markers, and ability for their secretome to augment a secondary cell population.

Results: The 3D hydrogel system resulted in an enhanced retention of more regenerative, nonsenescent ASC populations that exhibited increased expression of ‘stem-like’ MSC surface markers.

Conclusion: This study introduces a proof-of-concept design for a novel modular 3D system that can improve in vitro expansion of stem-like cell populations for future regenerative therapies.		en_US
dc.publisherFuture Science Groupen_US
dc.rightsCopyright 2022 The Authors. This work is licensed under the Attribution-NonCommercial-NoDerivatives 4.0 Unported License. To view a copy of this license.en_US
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0en_US
dc.subjectCell cultureen_US
dc.subjectHydrogelsen_US
dc.subjectRegenerative medicineen_US
dc.subjectStem cellsen_US
dc.subjectTissue engineeringen_US
dc.titleNovel hydrogel system eliminates subculturing and improves retention of nonsenescent mesenchymal stem cell populationsen_US
dc.typeArticleen_US
kusw.kuauthorHodge, Jacob G.
kusw.kuauthorRobinson, Jennifer L.
kusw.kudepartmentBioengineeringen_US
dc.identifier.doi10.2217/rme-2022-0140en_US
dc.identifier.orcidhttps://orcid.org/0000-0002-6165-3932en_US
kusw.oaversionScholarly/refereed, publisher versionen_US
kusw.oapolicyThis item meets KU Open Access policy criteria.en_US
dc.identifier.pmidPMC9732917en_US
dc.rights.accessrightsopenAccessen_US


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Copyright 2022 The Authors. This work is licensed under the Attribution-NonCommercial-NoDerivatives 4.0 Unported License. To view a copy of this license.
Except where otherwise noted, this item's license is described as: Copyright 2022 The Authors. This work is licensed under the Attribution-NonCommercial-NoDerivatives 4.0 Unported License. To view a copy of this license.