dc.contributor.author | Viriyaadhammaa, Natsima | |
dc.contributor.author | Duangmano, Suwit | |
dc.contributor.author | Saiai, Aroonchai | |
dc.contributor.author | Tungjai, Montree | |
dc.contributor.author | Dejkriengkraikul, Pornngarm | |
dc.contributor.author | Tima, Singkome | |
dc.contributor.author | Chiampanichayakul, Sawitree | |
dc.contributor.author | Krise, Jeffrey | |
dc.contributor.author | Anuchapreeda, Songyot | |
dc.date.accessioned | 2022-10-19T21:41:14Z | |
dc.date.available | 2022-10-19T21:41:14Z | |
dc.date.issued | 2022-08-12 | |
dc.identifier.citation | Viriyaadhammaa, N.; Duangmano, S.; Saiai, A.; Tungjai, M.; Dejkriengkraikul, P.; Tima, S.; Chiampanichayakul, S.; Krise, J.; Anuchapreeda, S. A Novel Drug Modulator Diarylheptanoid (trans-1,7-Diphenyl-5-hydroxy-1-heptene) from Curcuma comosa Rhizomes for P-glycoprotein Function and Apoptosis Induction in K652/ADR Leukemic Cells. Int. J. Mol. Sci. 2022, 23, 8989. https://doi.org/10.3390/ijms23168989 | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/33613 | |
dc.description.abstract | Curcuma comosa has been used in traditional Thai medicine to treat menstrual cycle-related symptoms in women. This study aims to evaluate the diarylheptanoid drug modulator, trans-1,7-diphenyl-5-hydroxy-1-heptene (DHH), in drug-resistant K562/ADR human leukemic cells. This compound was studied due to its effects on cell cytotoxicity, multidrug resistance (MDR) phenotype, P-glycoprotein (P-gp) expression, and P-gp function. We show that DHH itself is cytotoxic towards K562/ADR cells. However, DHH did not impact P-gp expression. The impact of DHH on the MDR phenotype in the K562/ADR cells was determined by co-treatment of cells with doxorubicin (Dox) and DHH using an MTT assay. The results showed that the DHH changed the MDR phenotype in the K562/ADR cells by decreasing the IC50 of Dox from 51.6 to 18.2 µM. Treating the cells with a nontoxic dose of DHH increased their sensitivity to Dox in P-gp expressing drug-resistant cells. The kinetics of P-gp mediated efflux of pirarubicin (THP) was used to monitor the P-gp function. DHH was shown to suppress THP efflux and resulted in enhanced apoptosis in the K562/ADR cells. These results demonstrate that DHH is a novel drug modulator of P-gp function and induces drug accumulation in the Dox-resistant K562 leukemic cell line. | en_US |
dc.publisher | MDPI | en_US |
dc.rights | © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license. | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en_US |
dc.subject | Curcuma comosa | en_US |
dc.subject | Diarylheptanoid | en_US |
dc.subject | trans-1,7-diphenyl-5-hydroxy-1-heptene | en_US |
dc.subject | Multidrug resistance | en_US |
dc.subject | MDR modulator | en_US |
dc.subject | Antileukemia | en_US |
dc.title | A Novel Drug Modulator Diarylheptanoid (trans-1,7-Diphenyl-5-hydroxy-1-heptene) from Curcuma comosa Rhizomes for P-glycoprotein Function and Apoptosis Induction in K652/ADR Leukemic Cells | en_US |
dc.type | Article | en_US |
kusw.kuauthor | Krise, Jeffrey | |
kusw.kudepartment | Pharmaceutical Chemistry | en_US |
dc.identifier.doi | 10.3390/ijms23168989 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0001-8732-8911 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-3452-8511 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0001-7259-4694 | en_US |
kusw.oaversion | Scholarly/refereed, publisher version | en_US |
kusw.oapolicy | This item meets KU Open Access policy criteria. | en_US |
dc.identifier.pmid | PMC9409401 | en_US |
dc.rights.accessrights | openAccess | en_US |