dc.contributor.author | Wijerathne, Harshani | |
dc.contributor.author | Witek, Malgorzata A. | |
dc.contributor.author | Jackson, Joshua M. | |
dc.contributor.author | Brown, Virginia | |
dc.contributor.author | Hupert, Mateusz L. | |
dc.contributor.author | Herrera, Kristina | |
dc.contributor.author | Kramer, Cameron | |
dc.contributor.author | Davidow, Abigail E. | |
dc.contributor.author | Li, Yan | |
dc.contributor.author | Baird, Alison E. | |
dc.contributor.author | Murphy, Michael C. | |
dc.contributor.author | Soper, Steven A. | |
dc.date.accessioned | 2022-09-20T18:23:07Z | |
dc.date.available | 2022-09-20T18:23:07Z | |
dc.date.issued | 2020-10-26 | |
dc.identifier.citation | Wijerathne, H., Witek, M.A., Jackson, J.M. et al. Affinity enrichment of extracellular vesicles from plasma reveals mRNA changes associated with acute ischemic stroke. Commun Biol 3, 613 (2020). https://doi.org/10.1038/s42003-020-01336-y | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/33550 | |
dc.description.abstract | Currently there is no in vitro diagnostic test for acute ischemic stroke (AIS), yet rapid diagnosis is crucial for effective thrombolytic treatment. We previously demonstrated the utility of CD8(+) T-cells’ mRNA expression for AIS detection; however extracellular vesicles (EVs) were not evaluated as a source of mRNA for AIS testing. We now report a microfluidic device for the rapid and efficient affinity-enrichment of CD8(+) EVs and subsequent EV’s mRNA analysis using droplet digital PCR (ddPCR). The microfluidic device contains a dense array of micropillars modified with anti-CD8α monoclonal antibodies that enriched 158 ± 10 nm sized EVs at 4.3 ± 2.1 × 109 particles/100 µL of plasma. Analysis of mRNA from CD8(+) EVs and their parental T-cells revealed correlation in the expression for AIS-specific genes in both cell lines and healthy donors. In a blinded study, 80% test positivity for AIS patients and controls was revealed with a total analysis time of 3.7 h. | en_US |
dc.publisher | Nature Research | en_US |
dc.rights | © The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License. | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en_US |
dc.subject | Mechanisms of disease | en_US |
dc.subject | Microfluidics | en_US |
dc.subject | Stroke | en_US |
dc.title | Affinity enrichment of extracellular vesicles from plasma reveals mRNA changes associated with acute ischemic stroke | en_US |
dc.type | Article | en_US |
kusw.kuauthor | Wijerathne, Harshani | |
kusw.kuauthor | Witek, Malgorzata A. | |
kusw.kuauthor | Jackson, Joshua M. | |
kusw.kuauthor | Brown, Virginia | |
kusw.kuauthor | Kramer, Cameron | |
kusw.kuauthor | Davidow, Abigail E. | |
kusw.kuauthor | Murphy, Michael C. | |
kusw.kuauthor | Soper, Steven A. | |
kusw.kudepartment | Chemistry | en_US |
kusw.kudepartment | Center of BioModular Multiscale Systems for Precision Medicine | en_US |
kusw.kudepartment | Bioengineering Program | en_US |
kusw.kudepartment | Mechanical Engineering | en_US |
dc.identifier.doi | 10.1038/s42003-020-01336-y | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-2802-7235 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-8292-7058 | en_US |
kusw.oaversion | Scholarly/refereed, publisher version | en_US |
kusw.oapolicy | This item meets KU Open Access policy criteria. | en_US |
dc.rights.accessrights | openAccess | en_US |