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Development of spirulina for the manufacture and oral delivery of protein therapeutics
dc.contributor.author | Jester, Benjamin W. | |
dc.contributor.author | Zhao, Hui | |
dc.contributor.author | Gewe, Mesfin | |
dc.contributor.author | Adame, Thomas | |
dc.contributor.author | Perruzza, Lisa | |
dc.contributor.author | Bolick, David T. | |
dc.contributor.author | Agosti, Jan | |
dc.contributor.author | Khuong, Nhi | |
dc.contributor.author | Kuestner, Rolf | |
dc.contributor.author | Gamble, Caitlin | |
dc.contributor.author | Cruickshank, Kendra | |
dc.contributor.author | Ferrara, Jeremy | |
dc.contributor.author | Lim, Rachelle | |
dc.contributor.author | Paddock, Troy | |
dc.contributor.author | Brady, Colin | |
dc.contributor.author | Ertel, Stacey | |
dc.contributor.author | Zhang, Miaohua | |
dc.contributor.author | Pollock, Alex | |
dc.contributor.author | Lee, Jamie | |
dc.contributor.author | Xiong, Jian | |
dc.contributor.author | Tasch, Michael | |
dc.contributor.author | Saveria, Tracy | |
dc.contributor.author | Doughty, David | |
dc.contributor.author | Marshall, Jacob | |
dc.contributor.author | Carrieri, Damian | |
dc.contributor.author | Goetsch, Lauren | |
dc.contributor.author | Dang, Jason | |
dc.contributor.author | Sanjaya, Nathaniel | |
dc.contributor.author | Fletcher, David | |
dc.contributor.author | Martinez, Anissa | |
dc.contributor.author | Kadis, Bryce | |
dc.contributor.author | Sigmar, Kristjan | |
dc.contributor.author | Afreen, Esha | |
dc.contributor.author | Nguyen, Tammy | |
dc.contributor.author | Randolph, Amanda | |
dc.contributor.author | Taber, Alexandria | |
dc.contributor.author | Krzeszowski, Ashley | |
dc.contributor.author | Robinett, Brittney | |
dc.contributor.author | Volkin, David B. | |
dc.contributor.author | Grassi, Fabio | |
dc.contributor.author | Guerrant, Richard | |
dc.contributor.author | Takeuchi, Ryo | |
dc.contributor.author | Finrow, Brian | |
dc.contributor.author | Behnke, Craig | |
dc.contributor.author | Roberts, James | |
dc.date.accessioned | 2022-07-12T19:46:51Z | |
dc.date.available | 2022-07-12T19:46:51Z | |
dc.date.issued | 2022-03-21 | |
dc.identifier.citation | Jester, B.W., Zhao, H., Gewe, M. et al. Development of spirulina for the manufacture and oral delivery of protein therapeutics. Nat Biotechnol 40, 956–964 (2022). https://doi.org/10.1038/s41587-022-01249-7 | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/32837 | |
dc.description.abstract | The use of the edible photosynthetic cyanobacterium Arthrospira platensis (spirulina) as a biomanufacturing platform has been limited by a lack of genetic tools. Here we report genetic engineering methods for stable, high-level expression of bioactive proteins in spirulina, including large-scale, indoor cultivation and downstream processing methods. Following targeted integration of exogenous genes into the spirulina chromosome (chr), encoded protein biopharmaceuticals can represent as much as 15% of total biomass, require no purification before oral delivery and are stable without refrigeration and protected during gastric transit when encapsulated within dry spirulina. Oral delivery of a spirulina-expressed antibody targeting campylobacter—a major cause of infant mortality in the developing world—prevents disease in mice, and a phase 1 clinical trial demonstrated safety for human administration. Spirulina provides an advantageous system for the manufacture of orally delivered therapeutic proteins by combining the safety of a food-based production host with the accessible genetic manipulation and high productivity of microbial platforms. | en_US |
dc.publisher | Nature Research | en_US |
dc.rights | This article is licensed under a Creative Commons Attribution 4.0 International License. | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en_US |
dc.subject | Genetic engineering | en_US |
dc.subject | Recombinant protein therapy | en_US |
dc.subject | Expression systems | en_US |
dc.subject | Protein delivery | en_US |
dc.title | Development of spirulina for the manufacture and oral delivery of protein therapeutics | en_US |
dc.type | Article | en_US |
kusw.kuauthor | Xiong, Jian | |
kusw.kuauthor | Volkin, David B. | |
kusw.kudepartment | Pharmaceutical Chemistry | en_US |
dc.identifier.doi | 10.1038/s41587-022-01249-7 | en_US |
dc.identifier.orcid | https://orcid.org/ 0000-0002-2997-7865 | en_US |
dc.identifier.orcid | https://orcid.org/ 0000-0002-4952-3297 | en_US |
dc.identifier.orcid | https://orcid.org/ 0000-0002-3978-299X | en_US |
dc.identifier.orcid | https://orcid.org/ 0000-0003-4026-3107 | en_US |
dc.identifier.orcid | https://orcid.org/ 0000-0002-0717-7496 | en_US |
dc.identifier.orcid | https://orcid.org/ 0000-0003-3348-1712 | en_US |
dc.identifier.orcid | https://orcid.org/ 0000-0001-7527-9307 | en_US |
dc.identifier.orcid | https://orcid.org/ 0000-0001-6949-7957 | en_US |
dc.identifier.orcid | https://orcid.org/ 0000-0003-3919-6760 | en_US |
kusw.oaversion | Scholarly/refereed, publisher version | en_US |
kusw.oapolicy | This item meets KU Open Access policy criteria. | en_US |
dc.identifier.pmid | PMC35314813 | en_US |
dc.rights.accessrights | openAccess | en_US |