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Strategies to Screen Anti-AQP4 Antibodies from Yeast Surface Display Libraries
dc.contributor.author | Huang, Aric | |
dc.contributor.author | Jin, Wei | |
dc.contributor.author | Fahad, Ahmed S. | |
dc.contributor.author | Mussman, Brooklyn K. | |
dc.contributor.author | Nicchia, Grazia Paola | |
dc.contributor.author | Madan, Bharat | |
dc.contributor.author | de Souza, Matheus Oliveira | |
dc.contributor.author | Griffin, J. Daniel | |
dc.contributor.author | Bennett, Jeffrey L. | |
dc.contributor.author | Frigeri, Antonio | |
dc.contributor.author | Berkland, Cory J. | |
dc.contributor.author | DeKosky, Brandon J. | |
dc.date.accessioned | 2022-07-12T19:23:47Z | |
dc.date.available | 2022-07-12T19:23:47Z | |
dc.date.issued | 2022-06-05 | |
dc.identifier.citation | Huang, A.; Jin, W.; Fahad, A.S.; Mussman, B.K.; Nicchia, G.P.; Madan, B.; de Souza, M.O.; Griffin, J.D.; Bennett, J.L.; Frigeri, A.; Berkland, C.J.; DeKosky, B.J. Strategies to Screen Anti-AQP4 Antibodies from Yeast Surface Display Libraries. Antibodies 2022, 11, 39. https://doi.org/10.3390/antib11020039 | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/32836 | |
dc.description.abstract | A rapid and effective method to identify disease-specific antibodies from clinical patients is important for understanding autoimmune diseases and for the development of effective disease therapies. In neuromyelitis optica (NMO), the identification of antibodies targeting the aquaporin-4 (AQP4) membrane protein traditionally involves the labor-intensive and time-consuming process of single B-cell sorting, followed by antibody cloning, expression, purification, and analysis for anti-AQP4 activity. To accelerate patient-specific antibody discovery, we compared two unique approaches for screening anti-AQP4 antibodies from yeast antibody surface display libraries. Our first approach, cell-based biopanning, has strong advantages for its cell-based display of native membrane-bound AQP4 antigens and is inexpensive and simple to perform. Our second approach, FACS screening using solubilized AQP4 antigens, permits real-time population analysis and precision sorting for specific antibody binding parameters. We found that both cell-based biopanning and FACS screening were effective for the enrichment of AQP4-binding clones. These screening techniques will enable library-scale functional interrogation of large natively paired antibody libraries for comprehensive analysis of anti-AQP4 antibodies in clinical samples and for robust therapeutic discovery campaigns. | en_US |
dc.publisher | MDPI | en_US |
dc.rights | © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license. | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en_US |
dc.subject | Yeast display | en_US |
dc.subject | Antibody discovery | en_US |
dc.subject | Yeast library screening | en_US |
dc.subject | Biopanning | en_US |
dc.subject | Aquaporin-4 | en_US |
dc.title | Strategies to Screen Anti-AQP4 Antibodies from Yeast Surface Display Libraries | en_US |
dc.type | Article | en_US |
kusw.kuauthor | Huang, Aric | |
kusw.kuauthor | Jin, Wei | |
kusw.kuauthor | Fahad, Ahmed S. | |
kusw.kuauthor | Mussman, Brooklyn K. | |
kusw.kuauthor | Madan, Bharat | |
kusw.kuauthor | de Souza, Matheus Oliveira | |
kusw.kuauthor | Griffin, J. Daniel | |
kusw.kuauthor | Berkland, Cory J. | |
kusw.kudepartment | Pharmaceutical Chemistry | en_US |
kusw.kudepartment | Chemical Engineering | en_US |
kusw.kudepartment | Bioengineering Graduate Program | en_US |
dc.identifier.doi | 10.3390/antib11020039 | en_US |
dc.identifier.orcid | https://orcid.org/ 0000-0001-8203-4818 | en_US |
dc.identifier.orcid | https://orcid.org/ 0000-0002-7376-9991 | en_US |
dc.identifier.orcid | https://orcid.org/ 0000-0001-6406-0836 | en_US |
kusw.oaversion | Scholarly/refereed, publisher version | en_US |
kusw.oapolicy | This item meets KU Open Access policy criteria. | en_US |
dc.identifier.pmid | PMC35735358 | en_US |
dc.rights.accessrights | openAccess | en_US |