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dc.contributor.authorTeng, I-Ting
dc.contributor.authorNazzari, Alexandra F.
dc.contributor.authorChoe, Misook
dc.contributor.authorLiu, Tracy
dc.contributor.authorde Souza, Matheus Oliveira
dc.contributor.authorPetrova, Yuliya
dc.contributor.authorTsybovsky, Yaroslav
dc.contributor.authorWang, Shuishu
dc.contributor.authorZhang, Baoshan
dc.contributor.authorArtamonov, Mykhaylo
dc.contributor.authorMadan, Bharat
dc.contributor.authorHuang, Aric
dc.contributor.authorLopez Acevedo, Sheila N.
dc.contributor.authorPan, Xiaoli
dc.contributor.authorRuckwardt, Tracy J.
dc.contributor.authorDeKosky, Brandon J.
dc.contributor.authorMascola, John R.
dc.contributor.authorMisasi, John
dc.contributor.authorSullivan, Nancy J.
dc.contributor.authorZhou, Tongqing
dc.contributor.authorKwong, Peter D.
dc.date.accessioned2022-07-05T19:23:35Z
dc.date.available2022-07-05T19:23:35Z
dc.date.issued2022-05-24
dc.identifier.citationTeng I-T, Nazzari AF, Choe M, Liu T, Oliveira de Souza M, Petrova Y, et al. (2022) Molecular probes of spike ectodomain and its subdomains for SARS-CoV-2 variants, Alpha through Omicron. PLoS ONE 17(5): e0268767. https://doi.org/10.1371/journal.pone.0268767en_US
dc.identifier.urihttp://hdl.handle.net/1808/32799
dc.description.abstractSince the outbreak of the COVID-19 pandemic, widespread infections have allowed SARS-CoV-2 to evolve in human, leading to the emergence of multiple circulating variants. Some of these variants show increased resistance to vaccine-elicited immunity, convalescent plasma, or monoclonal antibodies. In particular, mutations in the SARS-CoV-2 spike have drawn attention. To facilitate the isolation of neutralizing antibodies and the monitoring of vaccine effectiveness against these variants, we designed and produced biotin-labeled molecular probes of variant SARS-CoV-2 spikes and their subdomains, using a structure-based construct design that incorporated an N-terminal purification tag, a specific amino acid sequence for protease cleavage, the variant spike-based region of interest, and a C-terminal sequence targeted by biotin ligase. These probes could be produced by a single step using in-process biotinylation and purification. We characterized the physical properties and antigenicity of these probes, comprising the N-terminal domain (NTD), the receptor-binding domain (RBD), the RBD and subdomain 1 (RBD-SD1), and the prefusion-stabilized spike ectodomain (S2P) with sequences from SARS-CoV-2 variants of concern or of interest, including variants Alpha, Beta, Gamma, Epsilon, Iota, Kappa, Delta, Lambda, Mu, and Omicron. We functionally validated probes by using yeast expressing a panel of nine SARS-CoV-2 spike-binding antibodies and confirmed sorting capabilities of variant probes using yeast displaying libraries of plasma antibodies from COVID-19 convalescent donors. We deposited these constructs to Addgene to enable their dissemination. Overall, this study describes a matrix of SARS-CoV-2 variant molecular probes that allow for assessment of immune responses, identification of serum antibody specificity, and isolation and characterization of neutralizing antibodies.en_US
dc.publisherPublic Library of Scienceen_US
dc.rightsThis is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.en_US
dc.rights.urihttps://creativecommons.org/publicdomain/zero/1.0/en_US
dc.titleMolecular probes of spike ectodomain and its subdomains for SARS-CoV-2 variants, Alpha through Omicronen_US
dc.typeArticleen_US
kusw.kuauthorde Souza, Matheus Oliveira
kusw.kuauthorMadan, Bharat
kusw.kuauthorHuang, Aric
kusw.kuauthorLopez Acevedo, Sheila N.
kusw.kuauthorPan, Xiaoli
kusw.kuauthorDeKosky, Brandon J.
kusw.kudepartmentPharmaceutical Chemistryen_US
kusw.kudepartmentChemical Engineeringen_US
dc.identifier.doi10.1371/journal.pone.0268767en_US
dc.identifier.orcidhttps://orcid.org/ 0000-0003-0243-5203en_US
dc.identifier.orcidhttps://orcid.org/ 0000-0002-0865-4522en_US
dc.identifier.orcidhttps://orcid.org/ 0000-0002-0848-2054en_US
dc.identifier.orcidhttps://orcid.org/ 0000-0001-6406-0836en_US
dc.identifier.orcidhttps://orcid.org/ 0000-0001-7169-2639en_US
dc.identifier.orcidhttps://orcid.org/ 0000-0003-3560-232Xen_US
kusw.oaversionScholarly/refereed, publisher versionen_US
kusw.oapolicyThis item meets KU Open Access policy criteria.en_US
dc.identifier.pmidPMC35609088en_US
dc.rights.accessrightsopenAccessen_US


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This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.
Except where otherwise noted, this item's license is described as: This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.