Microfluidic Analysis of Exosomal Biomarkers towards Liquid Biopsy Diagnosis of Alzheimer's Disease

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Issue Date
2020-01-01Author
Zhang, Yaohua
Publisher
University of Kansas
Format
55 pages
Type
Thesis
Degree Level
M.S.
Discipline
Bioengineering
Rights
Copyright held by the author.
Metadata
Show full item recordAbstract
Alzheimer's Disease has posed a critical challenge to the global health system. Although no cure is currently available, early diagnosis is beneficial to both the health system and patients. To improve early diagnosis, a microfluidic platform is proposed to capture and detect neuronal exosomes. In this study, the microfluidic platform was first optimized and evaluated by a commercialized ELISA kit, and neuronal cell marker and neuronal exosomes were captured and analyzed on-chip by sandwich ELISA. The microchip achieved high sensitivity for both protein and exosome, which reaches 10pg/ml and 106 level respectively. Overall, this study illustrates the potentiality of the proposed microfluidic platform, which will be further improved to be applied for AD early diagnosis study.
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