Microfluidic Analysis of Exosomal Biomarkers towards Liquid Biopsy Diagnosis of Alzheimer's Disease

Abstract

Alzheimer's Disease has posed a critical challenge to the global health system. Although no cure is currently available, early diagnosis is beneficial to both the health system and patients. To improve early diagnosis, a microfluidic platform is proposed to capture and detect neuronal exosomes. In this study, the microfluidic platform was first optimized and evaluated by a commercialized ELISA kit, and neuronal cell marker and neuronal exosomes were captured and analyzed on-chip by sandwich ELISA. The microchip achieved high sensitivity for both protein and exosome, which reaches 10pg/ml and 106 level respectively. Overall, this study illustrates the potentiality of the proposed microfluidic platform, which will be further improved to be applied for AD early diagnosis study.