dc.contributor.author | Punchi Hewage, Achala N. D. | |
dc.contributor.author | Fontenot, Leo | |
dc.contributor.author | Guidry, Jessie | |
dc.contributor.author | Weldeghiorghis, Thomas | |
dc.contributor.author | Mehta, Anil K. | |
dc.contributor.author | Donnarumma, Fabrizio | |
dc.contributor.author | Rivera, Mario | |
dc.date.accessioned | 2022-01-17T19:27:03Z | |
dc.date.available | 2022-01-17T19:27:03Z | |
dc.date.issued | 2020-11-24 | |
dc.identifier.citation | Punchi Hewage, A.N.D.; Fontenot, L.; Guidry, J.; Weldeghiorghis, T.; Mehta, A.K.; Donnarumma, F.; Rivera, M. Mobilization of Iron Stored in Bacterioferritin Is Required for Metabolic Homeostasis in Pseudomonas aeruginosa. Pathogens 2020, 9, 980. https://doi.org/10.3390/pathogens9120980 | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/32409 | |
dc.description.abstract | Iron homeostasis offers a significant bacterial vulnerability because pathogens obtain essential iron from their mammalian hosts, but host-defenses maintain vanishingly low levels of free iron. Although pathogens have evolved mechanisms to procure host-iron, these depend on well-regulated iron homeostasis. To disrupt iron homeostasis, our work has targeted iron mobilization from the iron storage protein bacterioferritin (BfrB) by blocking a required interaction with its cognate ferredoxin partner (Bfd). The blockade of the BfrB–Bfd complex by deletion of the bfd gene (Δbfd) causes iron to irreversibly accumulate in BfrB. In this study we used mass spectrometry and NMR spectroscopy to compare the proteomic response and the levels of key intracellular metabolites between wild type (wt) and isogenic ΔbfdP. aeruginosa strains. We find that the irreversible accumulation of unusable iron in BfrB leads to acute intracellular iron limitation, even if the culture media is iron-sufficient. Importantly, the iron limitation and concomitant iron metabolism dysregulation trigger a cascade of events that lead to broader metabolic homeostasis disruption, which includes sulfur limitation, phenazine-mediated oxidative stress, suboptimal amino acid synthesis and altered carbon metabolism. | en_US |
dc.publisher | MDPI | en_US |
dc.rights | © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license. | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en_US |
dc.subject | Iron homeostasis | en_US |
dc.subject | Iron metabolism | en_US |
dc.subject | Sulfur metabolism | en_US |
dc.subject | Pseudomonas aeruginosa | en_US |
dc.subject | Ferritin | en_US |
dc.subject | Bacterioferritin | en_US |
dc.subject | Ferredoxin | en_US |
dc.subject | Proteomics | en_US |
dc.title | Mobilization of Iron Stored in Bacterioferritin Is Required for Metabolic Homeostasis in Pseudomonas aeruginosa | en_US |
dc.type | Article | en_US |
kusw.kuauthor | Punchi Hewage, Achala N. D. | |
kusw.kudepartment | Chemistry | en_US |
dc.identifier.doi | 10.3390/pathogens9120980 | en_US |
dc.identifier.orcid | https://orcid.org/ 0000-0003-3711-5162 | en_US |
dc.identifier.orcid | https://orcid.org/ 0000-0002-5601-0430 | en_US |
dc.identifier.orcid | https://orcid.org/ 0000-0003-1397-9919 | en_US |
dc.identifier.orcid | https://orcid.org/ 0000-0002-5692-5497 | en_US |
kusw.oaversion | Scholarly/refereed, publisher version | en_US |
kusw.oapolicy | This item meets KU Open Access policy criteria. | en_US |
dc.identifier.pmid | PMC7760384 | en_US |
dc.rights.accessrights | openAccess | en_US |