dc.contributor.author | Macey, J. Robert | |
dc.contributor.author | Pabinger, Stephan | |
dc.contributor.author | Barbieri, Charles G. | |
dc.contributor.author | Buring, Ella S. | |
dc.contributor.author | Gonzalez, Vanessa L. | |
dc.contributor.author | Mulcahy, Daniel G. | |
dc.contributor.author | DeMeo, Dustin P. | |
dc.contributor.author | Urban, Lara | |
dc.contributor.author | Hime, Paul M. | |
dc.contributor.author | Prost, Stefan | |
dc.contributor.author | Elliott, Aaron N. | |
dc.contributor.author | Gemmell, Neil J. | |
dc.date.accessioned | 2022-01-10T20:21:33Z | |
dc.date.available | 2022-01-10T20:21:33Z | |
dc.date.issued | 2021-01-29 | |
dc.identifier.citation | Macey, J.R., Pabinger, S., Barbieri, C.G. et al. Evidence of two deeply divergent co-existing mitochondrial genomes in the Tuatara reveals an extremely complex genomic organization. Commun Biol 4, 116 (2021). https://doi.org/10.1038/s42003-020-01639-0 | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/32381 | |
dc.description.abstract | Animal mitochondrial genomic polymorphism occurs as low-level mitochondrial heteroplasmy and deeply divergent co-existing molecules. The latter is rare, known only in bivalvian mollusks. Here we show two deeply divergent co-existing mt-genomes in a vertebrate through genomic sequencing of the Tuatara (Sphenodon punctatus), the sole-representative of an ancient reptilian Order. The two molecules, revealed using a combination of short-read and long-read sequencing technologies, differ by 10.4% nucleotide divergence. A single long-read covers an entire mt-molecule for both strands. Phylogenetic analyses suggest a 7–8 million-year divergence between genomes. Contrary to earlier reports, all 37 genes typical of animal mitochondria, with drastic gene rearrangements, are confirmed for both mt-genomes. Also unique to vertebrates, concerted evolution drives three near-identical putative Control Region non-coding blocks. Evidence of positive selection at sites linked to metabolically important transmembrane regions of encoded proteins suggests these two mt-genomes may confer an adaptive advantage for an unusually cold-tolerant reptile. | en_US |
dc.publisher | Nature Research | en_US |
dc.rights | Copyright © 2021, This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply. This article is licensed under a Creative Commons Attribution 4.0 International License. | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en_US |
dc.subject | Zoology | en_US |
dc.subject | Genomics | en_US |
dc.title | Evidence of two deeply divergent co-existing mitochondrial genomes in the Tuatara reveals an extremely complex genomic organization | en_US |
dc.type | Article | en_US |
kusw.kuauthor | Hime, Paul M. | |
kusw.kudepartment | Biodiversity Institute and Natural History Museum | en_US |
dc.identifier.doi | 10.1038/s42003-020-01639-0 | en_US |
dc.identifier.orcid | https://orcid.org/ 0000-0002-4198-956X | en_US |
dc.identifier.orcid | https://orcid.org/ 0000-0001-6614-2405 | en_US |
dc.identifier.orcid | https://orcid.org/ 0000-0002-5445-9314 | en_US |
dc.identifier.orcid | https://orcid.org/ 0000-0001-5322-4161 | en_US |
dc.identifier.orcid | https://orcid.org/ 0000-0002-6229-3596 | en_US |
dc.identifier.orcid | https://orcid.org/ 0000-0003-0671-3637 | en_US |
kusw.oaversion | Scholarly/refereed, publisher version | en_US |
kusw.oapolicy | This item meets KU Open Access policy criteria. | en_US |
dc.identifier.pmid | PMC7846811 | en_US |
dc.rights.accessrights | openAccess | en_US |