dc.contributor.author | Shelton, Claude | |
dc.date.accessioned | 2021-10-08T19:27:01Z | |
dc.date.available | 2021-10-08T19:27:01Z | |
dc.date.issued | 2007-08-31 | |
dc.identifier.uri | http://hdl.handle.net/1808/32100 | |
dc.description | Thesis (M.S.)--University of Kansas, Anatomy & Cell Biology, 2007. | en_US |
dc.description.abstract | To better understand SNS-Duf/Kirre mediated founder-fusion competent myoblast cell-cell recognition, we have addressed N-glycan requirements in SNS-Duf/Kirre mediated adhesion. Through site directed mutagenesis of N-glycosylation sites we have generated forms of SNS and Duf/Kirre lacking all N-glycans. Analysis of these mutants in S2 cell aggregation assays demonstrate a specific requirement for N-glycosylation of SNS in SNS-Duf/Kirre mediated aggregation, and for Duf/Kirre in Duf/Kirre-Duf/Kirre mediated aggregation. Since rescue experiments using SNS N-glycosylation mutants show only mild defects, it is apparent that, although the presence SNS is required for founder-fusion competent myoblast recognition, it is not the only molecule involved in proper execution of this process. | en_US |
dc.publisher | University of Kansas | en_US |
dc.rights | This item is protected by copyright and unless otherwise specified the copyright of this thesis/dissertation is held by the author. | en_US |
dc.subject | Biological sciences | en_US |
dc.title | Roles of N-linked glycosylation in SNS-Duf/Kirre mediated cell-cell adhesion | en_US |
dc.type | Thesis | en_US |
dc.thesis.degreeDiscipline | Anatomy & Cell Biology | |
dc.thesis.degreeLevel | M.S. | |
kusw.bibid | 6599241 | |
dc.rights.accessrights | openAccess | en_US |