Roles of N-linked glycosylation in SNS-Duf/Kirre mediated cell-cell adhesion
Issue Date
2007-08-31Author
Shelton, Claude
Publisher
University of Kansas
Type
Thesis
Degree Level
M.S.
Discipline
Anatomy & Cell Biology
Rights
This item is protected by copyright and unless otherwise specified the copyright of this thesis/dissertation is held by the author.
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Show full item recordAbstract
To better understand SNS-Duf/Kirre mediated founder-fusion competent myoblast cell-cell recognition, we have addressed N-glycan requirements in SNS-Duf/Kirre mediated adhesion. Through site directed mutagenesis of N-glycosylation sites we have generated forms of SNS and Duf/Kirre lacking all N-glycans. Analysis of these mutants in S2 cell aggregation assays demonstrate a specific requirement for N-glycosylation of SNS in SNS-Duf/Kirre mediated aggregation, and for Duf/Kirre in Duf/Kirre-Duf/Kirre mediated aggregation. Since rescue experiments using SNS N-glycosylation mutants show only mild defects, it is apparent that, although the presence SNS is required for founder-fusion competent myoblast recognition, it is not the only molecule involved in proper execution of this process.
Description
Thesis (M.S.)--University of Kansas, Anatomy & Cell Biology, 2007.
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- Theses [3943]
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