Electrochemical Measurement of Dopamine Release and Uptake in Zebrafish Following Treatment with Carboplatin
Field, Thomas M.
Stucky, Chase S.
Johnson, Michael A.
Scholarly/refereed, author accepted manuscript
© 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
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Post-chemotherapy cognitive impairment, also known as ‘chemobrain,’ is a neurological condition in which cognitive function is impaired as a result of cancer chemotherapy treatment. In this work, we used fast-scan cyclic voltammetry (FSCV) to measure electrically evoked dopamine release and uptake in whole brain preparations from zebrafish that have been treated with carboplatin, an agent associated with chemobrain. We administered carboplatin by addition to the fish's tank water or their food. One week of treatment with 100 μM carboplatin in the water was needed to significantly impair dopamine release (∼40 % of control); however, only one day of treatment through the zebrafish's food was needed to cause a similar impairment. Atomic absorption spectroscopy measurements suggested that administration through food resulted in higher initial levels of carboplatin compared to water administration, but water administration resulted in an increase over time. Uptake, determined by modeling stimulated release plots, was unaffected. These results are consistent with our previous findings of diminished neurotransmitter release in rats and support a role for zebrafish in chemobrain-related studies.
This is the peer reviewed version of the following article: T. M. Field, M. Shin, C. S. Stucky, J. Loomis, M. A. Johnson, ChemPhysChem 2018, 19, 1192., which has been published in final form at https://doi.org/10.1002/cphc.201701357. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.
T. M. Field, M. Shin, C. S. Stucky, J. Loomis, M. A. Johnson, ChemPhysChem 2018, 19, 1192.
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