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    Electrochemical Measurement of Dopamine Release and Uptake in Zebrafish Following Treatment with Carboplatin

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    Issue Date
    2018-03-23
    Author
    Field, Thomas M.
    Shin, Mimi
    Stucky, Chase S.
    Loomis, Joseph
    Johnson, Michael A.
    Publisher
    Wiley
    Type
    Article
    Article Version
    Scholarly/refereed, author accepted manuscript
    Rights
    © 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
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    Abstract
    Post-chemotherapy cognitive impairment, also known as ‘chemobrain,’ is a neurological condition in which cognitive function is impaired as a result of cancer chemotherapy treatment. In this work, we used fast-scan cyclic voltammetry (FSCV) to measure electrically evoked dopamine release and uptake in whole brain preparations from zebrafish that have been treated with carboplatin, an agent associated with chemobrain. We administered carboplatin by addition to the fish's tank water or their food. One week of treatment with 100 μM carboplatin in the water was needed to significantly impair dopamine release (∼40 % of control); however, only one day of treatment through the zebrafish's food was needed to cause a similar impairment. Atomic absorption spectroscopy measurements suggested that administration through food resulted in higher initial levels of carboplatin compared to water administration, but water administration resulted in an increase over time. Uptake, determined by modeling stimulated release plots, was unaffected. These results are consistent with our previous findings of diminished neurotransmitter release in rats and support a role for zebrafish in chemobrain-related studies.
    Description
    This is the peer reviewed version of the following article: T. M. Field, M. Shin, C. S. Stucky, J. Loomis, M. A. Johnson, ChemPhysChem 2018, 19, 1192., which has been published in final form at https://doi.org/10.1002/cphc.201701357. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.
    URI
    http://hdl.handle.net/1808/31869
    DOI
    https://doi.org/10.1002/cphc.201701357
    Collections
    • Chemistry Scholarly Works [610]
    Citation
    T. M. Field, M. Shin, C. S. Stucky, J. Loomis, M. A. Johnson, ChemPhysChem 2018, 19, 1192.

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    Lawrence, KS 66045
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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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