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dc.contributor.authorArizmendi, Olivia
dc.contributor.authorKumar, Prashant
dc.contributor.authorZheng, Qi
dc.contributor.authorStewart, Jason P.
dc.contributor.authorPicking, William D.
dc.contributor.authorPicking, Wendy
dc.contributor.authorMartinez-Becerra, Francisco J.
dc.identifier.citationArizmendi O, Kumar P, Zheng Q, Stewart JP, Picking WD, Picking W and Martinez-Becerra FJ (2019) Vaccination With Mouse Dendritic Cells Loaded With an IpaD-IpaB Fusion Provides Protection Against Shigellosis. Front. Immunol. 10:192. doi: 10.3389/fimmu.2019.00192en_US
dc.descriptionThis work is licensed under a Creative Commons Attribution 4.0 International License.en_US
dc.description.abstractDiarrheal diseases are a major cause of morbidity and mortality worldwide. They are most prevalent in settings with inadequate sanitation, poor hygiene and contaminated water. An important diarrheal pathogen in such settings is Shigella. No commercially available vaccine exists against shigellosis and immunity to the pathogen is serotype-restricted. We have previously shown that a polypeptide fusion of the Type Three Secretion Apparatus (T3SA) proteins IpaB and IpaD (named DBF) was efficacious as a vaccine against Shigella. Vaccination using different administration routes indicated that protection conferred by DBF did not fully correlate with antibodies. To define the immune responses involved in protection, we studied cellular responses to intranasal immunization with the DBF and the adjuvant dmLT. We found dendritic cell (DC) activation at the nasal associated lymphoid tissue (NALT). Activation markers CD86 and MHCII significantly increase in cells from immunized mice. Antigen exposure in vitro further confirmed the upregulation of CD80 and CD40 in primary dendritic cells. Animals immunized with antigen-primed dendritic cells were protected against Shigella infection, at levels comparable to the efficacy of immunization with the protein vaccine formulation. Therefore, we show that antigen-primed DCs are enough to provide immunity, and propose a mechanism of protection against Shigella spp. based on DC-mediated antigen presentation to T cells.en_US
dc.publisherFrontiers Mediaen_US
dc.rightsCopyright © 2019 Arizmendi, Kumar, Zheng, Stewart, Picking, Picking and Martinez-Becerra.en_US
dc.subjectFusion proteinen_US
dc.subjectDendritic cellen_US
dc.titleVaccination With Mouse Dendritic Cells Loaded With an IpaD-IpaB Fusion Provides Protection Against Shigellosisen_US
kusw.kuauthorArizmendi, Olivia
kusw.kuauthorKumar, Prashant
kusw.kuauthorZheng, Qi
kusw.kuauthorStewart, Jason P.
kusw.kuauthorPicking, William D.
kusw.kuauthorPicking, Wendy
kusw.kuauthorMartinez-Becerra, Francisco J.
kusw.kudepartmentPharmaceutical Chemistryen_US
kusw.kudepartmentHiguchi Biosciences Centeren_US
kusw.oaversionScholarly/refereed, publisher versionen_US
kusw.oapolicyThis item meets KU Open Access policy criteria.en_US

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Copyright © 2019 Arizmendi, Kumar, Zheng, Stewart, Picking, Picking and Martinez-Becerra.
Except where otherwise noted, this item's license is described as: Copyright © 2019 Arizmendi, Kumar, Zheng, Stewart, Picking, Picking and Martinez-Becerra.