Show simple item record

dc.contributor.authorHu, Qingting
dc.contributor.authorMyers, Megan
dc.contributor.authorFang, Wei
dc.contributor.authorYao, Min
dc.contributor.authorBrummer, Gage
dc.contributor.authorHawj, Justin
dc.contributor.authorSmart, Curtis
dc.contributor.authorBerkland, Cory
dc.contributor.authorCheng, Nikki
dc.identifier.citationHu, Q., Myers, M., Fang, W., Yao, M., Brummer, G., Hawj, J., Smart, C., Berkland, C., & Cheng, N. (2019). Role of ALDH1A1 and HTRA2 expression in CCL2/CCR2-mediated breast cancer cell growth and invasion. Biology open, 8(7), bio040873.
dc.descriptionThis work is licensed under a Creative Commons Attribution 4.0 International License.en_US
dc.description.abstractChemokines mediate immune cell trafficking during tissue development, wound healing and infection. The chemokine CCL2 is best known to regulate macrophage recruitment during wound healing, infection and inflammatory diseases. While the importance of CCL2/CCR2 signaling in macrophages during cancer progression is well documented, we recently showed that CCL2-mediated breast cancer progression depends on CCR2 expression in carcinoma cells. Using 3D Matrigel: Collagen cultures of SUM225 and breast cancer cells, this study characterized the mechanisms of CCL2/CCR2 signaling in cell growth and invasion. SUM225 cells, which expressed lower levels of CCR2 than cells, formed symmetrical spheroids in Matrigel: Collagen, and were not responsive to CCL2 treatment. cells formed asymmetric cell clusters in Matrigel: Collagen. CCL2 treatment increased growth, decreased expression of E-cadherin and increased TWIST1 expression. CCR2 overexpression in SUM225 cells increased responsiveness to CCL2 treatment, enhancing growth and invasion. These phenotypes corresponded to increased expression of Aldehyde Dehydrogenase 1A1 (ALDH1A1) and decreased expression of the mitochondrial serine protease HTRA2. CCR2 deficiency in cells inhibited CCL2-mediated growth and invasion, corresponding to decreased ALDH1A1 expression and increased HTRA2 expression. ALDH1A1 and HTRA2 expression were modulated in CCR2-deficient and CCR2-overexpressing cell lines. We found that ALDH1A1 and HTRA2 regulates CCR2-mediated breast cancer cell growth and cellular invasion in a CCL2/CCR2 context-dependent manner. These data provide novel insight on the mechanisms of chemokine signaling in breast cancer cell growth and invasion, with important implications on targeted therapeutics for anti-cancer treatment.en_US
dc.description.sponsorshipSusan G. Komen Foundation (CCR13261859)en_US
dc.description.sponsorshipNIH CA172764en_US
dc.publisherThe Company of Biologistsen_US
dc.rights© 2019. Published by The Company of Biologists Ltden_US
dc.subjectBreast canceren_US
dc.subject3D cultureen_US
dc.subjectCell invasionen_US
dc.titleRole of ALDH1A1 and HTRA2 expression in CCL2/CCR2-mediated breast cancer cell growth and invasionen_US
kusw.kuauthorBerkland, Cory
kusw.kudepartmentPharmaceutical Chemistryen_US
kusw.oanotesPer Sherpa Romeo 11/17/2020:

Biology Open [Open panel below]Publication Information TitleBiology Open [English] ISSNsElectronic: 2046-6390 URL PublishersThe Company of Biologists [Society Publisher] DOAJ Listing Requires APCYes [Data provided by DOAJ] [Open panel below]Publisher Policy Open Access pathways permitted by this journal's policy are listed below by article version. Click on a pathway for a more detailed view.

Published Version NoneCC BYPMC Any Website, +1 EmbargoNo Embargo LicenceCC BY Publisher DepositPubMed Central Location Any Repository Any Website ConditionsMust link to publisher version
kusw.oaversionScholarly/refereed, publisher versionen_US
kusw.oapolicyThis item meets KU Open Access policy criteria.en_US

Files in this item


This item appears in the following Collection(s)

Show simple item record

© 2019. Published by The Company of Biologists Ltd
Except where otherwise noted, this item's license is described as: © 2019. Published by The Company of Biologists Ltd