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dc.contributor.authorNorouzi, Mohammad
dc.contributor.authorYathindranath, Vinith
dc.contributor.authorThliveris, James A.
dc.contributor.authorKopec, Brian M.
dc.contributor.authorSiahaan, Teruna J.
dc.contributor.authorMiller, Donald W.
dc.date.accessioned2020-11-12T16:59:07Z
dc.date.available2020-11-12T16:59:07Z
dc.date.issued2020-07-09
dc.identifier.citationNorouzi, M., Yathindranath, V., Thliveris, J. A., Kopec, B. M., Siahaan, T. J., & Miller, D. W. (2020). Doxorubicin-loaded iron oxide nanoparticles for glioblastoma therapy: a combinational approach for enhanced delivery of nanoparticles. Scientific reports, 10(1), 11292. https://doi.org/10.1038/s41598-020-68017-yen_US
dc.identifier.urihttp://hdl.handle.net/1808/30845
dc.descriptionThis work is licensed under a Creative Commons Attribution 4.0 International License.en_US
dc.description.abstractAlthough doxorubicin (DOX) is an effective anti-cancer drug with cytotoxicity in a variety of different tumors, its effectiveness in treating glioblastoma multiforme (GBM) is constrained by insufficient penetration across the blood–brain barrier (BBB). In this study, biocompatible magnetic iron oxide nanoparticles (IONPs) stabilized with trimethoxysilylpropyl-ethylenediamine triacetic acid (EDT) were developed as a carrier of DOX for GBM chemotherapy. The DOX-loaded EDT-IONPs (DOX-EDT-IONPs) released DOX within 4 days with the capability of an accelerated release in acidic microenvironments. The DOX-loaded EDT-IONPs (DOX-EDT-IONPs) demonstrated an efficient uptake in mouse brain-derived microvessel endothelial, bEnd.3, Madin–Darby canine kidney transfected with multi-drug resistant protein 1 (MDCK-MDR1), and human U251 GBM cells. The DOX-EDT-IONPs could augment DOX’s uptake in U251 cells by 2.8-fold and significantly inhibited U251 cell proliferation. Moreover, the DOX-EDT-IONPs were found to be effective in apoptotic-induced GBM cell death (over 90%) within 48 h of treatment. Gene expression studies revealed a significant downregulation of TOP II and Ku70, crucial enzymes for DNA repair and replication, as well as MiR-155 oncogene, concomitant with an upregulation of caspase 3 and tumor suppressors i.e., p53, MEG3 and GAS5, in U251 cells upon treatment with DOX-EDT-IONPs. An in vitro MDCK-MDR1-GBM co-culture model was used to assess the BBB permeability and anti-tumor activity of the DOX-EDT-IONPs and DOX treatments. While DOX-EDT-IONP showed improved permeability of DOX across MDCK-MDR1 monolayers compared to DOX alone, cytotoxicity in U251 cells was similar in both treatment groups. Using a cadherin binding peptide (ADTC5) to transiently open tight junctions, in combination with an external magnetic field, significantly enhanced both DOX-EDT-IONP permeability and cytotoxicity in the MDCK-MDR1-GBM co-culture model. Therefore, the combination of magnetic enhanced convective diffusion and the cadherin binding peptide for transiently opening the BBB tight junctions are expected to enhance the efficacy of GBM chemotherapy using the DOX-EDT-IONPs. In general, the developed approach enables the chemotherapeutic to overcome both BBB and multidrug resistance (MDR) glioma cells while providing site-specific magnetic targeting.en_US
dc.description.sponsorshipCanadian Institutes of Health Researchen_US
dc.description.sponsorshipNatural Science and Engineering Research Council—Canadaen_US
dc.description.sponsorshipNIH R01-NS075374en_US
dc.description.sponsorshipNIH P30-AG035982en_US
dc.description.sponsorshipNIH T32-GM008359en_US
dc.publisherNature Researchen_US
dc.rightsCopyright © 2020, The Author(s)en_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.subjectNanobiotechnologyen_US
dc.subjectNanoscale materialsen_US
dc.titleDoxorubicin-loaded iron oxide nanoparticles for glioblastoma therapy: A combinational approach for enhanced delivery of nanoparticlesen_US
dc.typeArticleen_US
kusw.kuauthorKopec, Brian M.
kusw.kuauthorSiahaan, Teruna J.
kusw.kudepartmentPharmaceutical Chemistryen_US
dc.identifier.doi10.1038/s41598-020-68017-yen_US
kusw.oaversionScholarly/refereed, publisher versionen_US
kusw.oapolicyThis item meets KU Open Access policy criteria.en_US
dc.identifier.pmidPMC7347880en_US
dc.rights.accessrightsopenAccessen_US


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