dc.contributor.author | Caruso, Giuseppe | |
dc.contributor.author | Musso, Nicolò | |
dc.contributor.author | Grasso, Margherita | |
dc.contributor.author | Costantino, Angelita | |
dc.contributor.author | Lazzarino, Giuseppe | |
dc.contributor.author | Tascedda, Fabio | |
dc.contributor.author | Gulisano, Massimo | |
dc.contributor.author | Lunte, Susan M. | |
dc.contributor.author | Caraci, Filippo | |
dc.date.accessioned | 2020-11-12T15:53:21Z | |
dc.date.available | 2020-11-12T15:53:21Z | |
dc.date.issued | 2020-06-15 | |
dc.identifier.citation | Caruso, G., Musso, N., Grasso, M., Costantino, A., Lazzarino, G., Tascedda, F., Gulisano, M., Lunte, S. M., & Caraci, F. (2020). Microfluidics as a Novel Tool for Biological and Toxicological Assays in Drug Discovery Processes: Focus on Microchip Electrophoresis. Micromachines, 11(6), 593. https://doi.org/10.3390/mi11060593 | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/30841 | |
dc.description | This work is licensed under a Creative Commons Attribution 4.0 International License. | en_US |
dc.description.abstract | The last decades of biological, toxicological, and pharmacological research have deeply changed the way researchers select the most appropriate ‘pre-clinical model’. The absence of relevant animal models for many human diseases, as well as the inaccurate prognosis coming from ‘conventional’ pre-clinical models, are among the major reasons of the failures observed in clinical trials. This evidence has pushed several research groups to move more often from a classic cellular or animal modeling approach to an alternative and broader vision that includes the involvement of microfluidic-based technologies. The use of microfluidic devices offers several benefits including fast analysis times, high sensitivity and reproducibility, the ability to quantitate multiple chemical species, and the simulation of cellular response mimicking the closest human in vivo milieu. Therefore, they represent a useful way to study drug–organ interactions and related safety and toxicity, and to model organ development and various pathologies ‘in a dish’. The present review will address the applicability of microfluidic-based technologies in different systems (2D and 3D). We will focus our attention on applications of microchip electrophoresis (ME) to biological and toxicological studies as well as in drug discovery and development processes. These include high-throughput single-cell gene expression profiling, simultaneous determination of antioxidants and reactive oxygen and nitrogen species, DNA analysis, and sensitive determination of neurotransmitters in biological fluids. We will discuss new data obtained by ME coupled to laser-induced fluorescence (ME-LIF) and electrochemical detection (ME-EC) regarding the production and degradation of nitric oxide, a fundamental signaling molecule regulating virtually every critical cellular function. Finally, the integration of microfluidics with recent innovative technologies—such as organoids, organ-on-chip, and 3D printing—for the design of new in vitro experimental devices will be presented with a specific attention to drug development applications. This ‘composite’ review highlights the potential impact of 2D and 3D microfluidic systems as a fast, inexpensive, and highly sensitive tool for high-throughput drug screening and preclinical toxicological studies. | en_US |
dc.description.sponsorship | Italian Ministry of Health Research Program 2018 (2635256) | en_US |
dc.description.sponsorship | American Heart Association-Midwest Affiliate Postdoctoral Research Fellowship (NFP0075515) | en_US |
dc.description.sponsorship | Italian Ministry of Economic Development (F/200110/02/X45) | en_US |
dc.description.sponsorship | Italian Ministry of Education | en_US |
dc.description.sponsorship | NIH COBRE P20GM103638 | en_US |
dc.description.sponsorship | Oasi Research Institute—IRCCS | en_US |
dc.publisher | MDPI | en_US |
dc.rights | © 2020 by the authors. Licensee MDPI, Basel, Switzerland. | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en_US |
dc.subject | Toxicology | en_US |
dc.subject | Drug screening | en_US |
dc.subject | Microchip electrophoresis | en_US |
dc.subject | Carnosine | en_US |
dc.subject | Organs-on-a-chip | en_US |
dc.subject | Organoids | en_US |
dc.subject | 3D bioprinting | en_US |
dc.title | Microfluidics as a Novel Tool for Biological and Toxicological Assays in Drug Discovery Processes: Focus on Microchip Electrophoresis | en_US |
dc.type | Article | en_US |
kusw.kuauthor | Lunte, Susan M. | |
kusw.kudepartment | Pharmaceutical Chemistry | en_US |
kusw.kudepartment | Ralph N. Adams Institute for Bioanalytical Chemistry | en_US |
kusw.kudepartment | Chemistry | en_US |
dc.identifier.doi | 10.3390/mi11060593 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0003-1571-5327 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0003-2451-1158 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-5917-7279 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-0958-7333 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0001-8654-1745 | en_US |
kusw.oaversion | Scholarly/refereed, publisher version | en_US |
kusw.oapolicy | This item meets KU Open Access policy criteria. | en_US |
dc.identifier.pmid | PMC7344675 | en_US |
dc.rights.accessrights | openAccess | en_US |