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dc.contributor.authorYazici, Hilal
dc.contributor.authorHabib, Gizem
dc.contributor.authorBoone, Kyle
dc.contributor.authorUrgen, Mustafa
dc.contributor.authorUtku, Feride Sermin
dc.contributor.authorTamerler, Candan
dc.identifier.citationYazici, H., Habib, G., Boone, K., Urgen, M., Utku, F. S., & Tamerler, C. (2019). Self-assembling antimicrobial peptides on nanotubular titanium surfaces coated with calcium phosphate for local therapy. Materials science & engineering. C, Materials for biological applications, 94, 333–343.
dc.descriptionThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.en_US
dc.description.abstractBacterial infection is a serious medical problem leading to implant failure. The current antibiotic based therapies rise concerns due to bacterial resistance. The family of antimicrobial peptides (AMP) is one of the promising candidates as local therapy agents due to their broad-spectrum activity. Despite AMPs receive increasing attention to treat infection, their effective delivery to the implantation site has been limited. Here, we developed an engineered dual functional peptide which delivers AMP as a biomolecular therapeutic agent onto calcium phosphate deposited nanotubular titanium surfaces. Dual functionality of the peptide was achieved by combining a hydroxyapatite binding peptide-1 (HABP1) with an AMP using a flexible linker. HABP functionality of the peptide provided a self-coating property onto the nano-topographies that are designed to improve osteointegration capability, while AMP offered an antimicrobial protection onto the implant surface. We successfully deposited calcium phosphate minerals on nanotubular titanium oxide surface using pulse electrochemical deposition (PECD) and characterized the minerals by XRD, FT-IR, FE-SEM. Antimicrobial activity of the engineered peptide was tested against S. mutans (gram- positive) and E. coli (gram-negative) both in solution and on the Ca-P coated nanotubular titanium surface. In solution activity of AMP and dual functional peptide have the same Minimum Inhibitory Concentration (MIC) (32 mg/mL) against E.coli. The peptide also resulted in the reduction of the number of bacteria both for E.coli and S.mutans compare to control groups. Antimicrobial features of dual functional peptides are strongly correlated with their structures suggesting tunability in design through linkers regions. The dual-function peptide offers single-step solution for implant surface functionalization that could be applicable to any implant surface having different topographies.en_US
dc.description.sponsorshipNIH AR062249–03en_US
dc.description.sponsorshipNIH R01DE025476–01en_US
dc.description.sponsorshipTUBITAK BIDEP 2218en_US
dc.description.sponsorshipITU Institute for Graduate Programsen_US
dc.rights© 2018 Elsevier B.V. All rights reserved.en_US
dc.subjectImplant infectionsen_US
dc.subjectHydroxyapatite-binding peptideen_US
dc.subjectCalcium-phosphate coatingsen_US
dc.subjectNanotubular titaniumen_US
dc.subjectSelf-assembled peptidesen_US
dc.subjectAntimicrobial peptidesen_US
dc.titleSelf-assembling antimicrobial peptides on nanotubular titanium surfaces coated with calcium phosphate for local therapyen_US
kusw.kuauthorBoone, Kyle
kusw.kuauthorTamerler, Candan
kusw.kudepartmentMechanical Engineeringen_US
kusw.oaversionScholarly/refereed, author accepted manuscripten_US
kusw.oapolicyThis item meets KU Open Access policy criteria.en_US

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© 2018 Elsevier B.V. All rights reserved.
Except where otherwise noted, this item's license is described as: © 2018 Elsevier B.V. All rights reserved.