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dc.contributor.authorWisdom, E. Cate
dc.contributor.authorZhou, Yan
dc.contributor.authorChen, Casey
dc.contributor.authorTamerler, Candan
dc.contributor.authorSnead, Malcolm L.
dc.date.accessioned2020-10-20T15:55:13Z
dc.date.available2020-10-20T15:55:13Z
dc.date.issued2019-09-24
dc.identifier.citationWisdom, E. C., Zhou, Y., Chen, C., Tamerler, C., & Snead, M. L. (2020). Mitigation of peri-implantitis by rational design of bifunctional peptides with antimicrobial properties. ACS biomaterials science & engineering, 6(5), 2682–2695. https://doi.org/10.1021/acsbiomaterials.9b01213en_US
dc.identifier.urihttp://hdl.handle.net/1808/30784
dc.descriptionThis document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Biomaterials Science and Engineering, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acsbiomaterials.9b01213.en_US
dc.description.abstractThe integration of molecular and cell biology with materials science has led to strategies to improve the interface between dental implants with the surrounding soft and hard tissues in order to replace missing teeth and restore mastication. More than 3 million implants have been placed in the US alone and this number is rising by 500,000/year. Peri-implantitis, an inflammatory response to oral pathogens growing on the implant surface threatens to reduce service life leading to eventual implant failure, and such an outcome will have adverse impact on public health and create significant health care costs. Here we report a predictive approach to peptide design, which enabled us to engineer a bifunctional peptide to combat bacterial colonization and biofilm formation, reducing the adverse host inflammatory immune response that destroys the tissue surrounding implants and shortens their lifespans. This bifunctional peptide contains a titanium-binding domain that recognizes and binds with high affinity to titanium implant surfaces, fused through a rigid spacer domain with an antimicrobial domain. By varying the antimicrobial peptide domain, we were able to predict the properties of the resulting bifunctional peptides in their entirety by analyzing the sequence-structure-function relationship. These bifunctional peptides achieve: 1) nearly 100% surface coverage within minutes, a timeframe suitable for their clinical application to existing implants; 2) nearly 100% binding to a titanium surface even in the presence of contaminating serum protein; 3) durability to brushing with a commercially available electric toothbrush; and 4) retention of antimicrobial activity on the implant surface following bacterial challenge. A bifunctional peptide film can be applied to both new implants and/or repeatedly applied to previously placed implants to control bacterial colonization mitigating peri-implant disease that threatens dental implant longevity.en_US
dc.publisherAmerican Chemical Societyen_US
dc.rightsCopyright © 2019 American Chemical Societyen_US
dc.subjectPeri-implant diseaseen_US
dc.subjectBifunctional Peptidesen_US
dc.subjectAntimicrobialen_US
dc.subjectStructureen_US
dc.subjectFunctionen_US
dc.subjectTitanium/Ti Alloy Implanten_US
dc.subjectDental Implanten_US
dc.subjectStabilityen_US
dc.subjectPeptide Filmen_US
dc.titleMitigation of peri-implantitis by rational design of bifunctional peptides with antimicrobial propertiesen_US
dc.typeArticleen_US
kusw.kuauthorWisdom, E. Cate
kusw.kuauthorTamerler, Candan
kusw.kudepartmentBioengineering Research Centeren_US
kusw.kudepartmentMechanical Engineeringen_US
dc.identifier.doi10.1021/acsbiomaterials.9b01213en_US
dc.identifier.orcidhttp://orcid.org/0000-0002-4496-6478en_US
kusw.oaversionScholarly/refereed, author accepted manuscripten_US
kusw.oapolicyThis item meets KU Open Access policy criteria.en_US
dc.identifier.pmidPMC7255409en_US
dc.rights.accessrightsopenAccessen_US


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