dc.contributor.advisor | Gallagher, Phillip | |
dc.contributor.author | Landes, Evan | |
dc.date.accessioned | 2019-06-12T02:56:49Z | |
dc.date.available | 2019-06-12T02:56:49Z | |
dc.date.issued | 2018-05-31 | |
dc.date.submitted | 2018 | |
dc.identifier.other | http://dissertations.umi.com/ku:16026 | |
dc.identifier.uri | http://hdl.handle.net/1808/29309 | |
dc.description.abstract | The primary aim of this study is to determine if orange juice (OJ) supplementation can attenuate cytokine (IL-6 and TNF-α) levels in human skeletal muscle following exercise in the heat. We hypothesized that exercise in a hot humid environment will increase IL-6 and TNF-α levels in skeletal muscle. Secondly we hypothesized that supplementation with 100% OJ will partially attenuate the cytokine response to exercise in a similar manner to carbohydrate-electrolyte beverage (CEB) but more so than just drinking water (W) following exercise. In a single blind fashion, twenty healthy males and six healthy females, consumed either 100% OJ, W, or a CEB for eleven days. Over five of those days they performed 80 minutes of intermittent cycling (4 sets of 15min at 70% max heart rate) in an environmental chamber set at a mild, thermal temperature (30°C, 50% humidity). Muscle biopsies were taken from vastus lateralis on days 1, 4, 8, and 11 of the study and analyzed for the cytokines IL-6 and TNF-α via Western Blot protein immunoblotting technique. There was no main effect for either time or group IL-6 in skeletal muscle. However, there was a group x time interaction (p=0.018), where the group that consumed W linearly increased from day 1 to day 11 while the OJ, and CEB groups did not change. Post-hoc independent T-tests found a significant difference between the OJ and W group on Day 8 (p=0.028). TNF-α skeletal muscle levels were below the detection level of the imaging system and were not analyzed statisctically. In conclusion, there was no significant difference in intramuscular IL-6 between groups that consumed OJ, CEB, or W after repeated bouts of aerobic exercise. However, our data does suggest that the carbohydrate content of OJ and CEB could plausibly attenuate IL-6 increase when compared to just W. | |
dc.format.extent | 47 pages | |
dc.language.iso | en | |
dc.publisher | University of Kansas | |
dc.rights | Copyright held by the author. | |
dc.subject | Physiology | |
dc.subject | Cycling | |
dc.subject | Cytokines | |
dc.subject | Interleukin-6 | |
dc.subject | Orange Juice | |
dc.subject | Skeletal Muscle | |
dc.subject | Tumor Necrosis Factor-Alpha | |
dc.title | Skeletal Muscle Cytokines following Repeated Bouts of Exercise and Orange Juice | |
dc.type | Thesis | |
dc.contributor.cmtemember | Herda, Ashley | |
dc.contributor.cmtemember | Emerson, Dawn | |
dc.thesis.degreeDiscipline | Health, Sport and Exercise Sciences | |
dc.thesis.degreeLevel | M.S.Ed. | |
dc.identifier.orcid | | |
dc.rights.accessrights | openAccess | |