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dc.contributor.authorWitek, Małgorzata A.
dc.contributor.authorAufforth, Rachel D.
dc.contributor.authorWang, Hong
dc.contributor.authorKamande, Joyce W.
dc.contributor.authorJackson, Joshua M.
dc.contributor.authorPullagurla, Swathi R.
dc.contributor.authorHupert, Mateusz L.
dc.contributor.authorUsary, Jerry
dc.contributor.authorWysham, Weiya Z.
dc.contributor.authorHilliard, Dawud
dc.contributor.authorMontgomery, Stephanie
dc.contributor.authorBae-Jump, Victoria
dc.contributor.authorCarey, Lisa A.
dc.contributor.authorGehrig, Paola A.
dc.contributor.authorMilowsky, Matthew I.
dc.contributor.authorPerou, Charles M.
dc.contributor.authorSoper, John T.
dc.contributor.authorWhang, Young E.
dc.contributor.authorYeh, Jen Jen
dc.contributor.authorGeorge Martin, George
dc.contributor.authorSoper, Steven A.
dc.date.accessioned2018-05-21T17:50:17Z
dc.date.available2018-05-21T17:50:17Z
dc.date.issued2017-07-25
dc.identifier.citationWitek, M. A., Aufforth, R. D., Wang, H., Kamande, J. W., Jackson, J. M., Pullagurla, S. R., … Soper, S. A. (2017). Discrete microfluidics for the isolation of circulating tumor cell subpopulations targeting fibroblast activation protein alpha and epithelial cell adhesion molecule. NPJ Precision Oncology, 1, 24. http://doi.org/10.1038/s41698-017-0028-8en_US
dc.identifier.urihttp://hdl.handle.net/1808/26431
dc.description.abstractCirculating tumor cells consist of phenotypically distinct subpopulations that originate from the tumor microenvironment. We report a circulating tumor cell dual selection assay that uses discrete microfluidics to select circulating tumor cell subpopulations from a single blood sample; circulating tumor cells expressing the established marker epithelial cell adhesion molecule and a new marker, fibroblast activation protein alpha, were evaluated. Both circulating tumor cell subpopulations were detected in metastatic ovarian, colorectal, prostate, breast, and pancreatic cancer patients and 90% of the isolated circulating tumor cells did not co-express both antigens. Clinical sensitivities of 100% showed substantial improvement compared to epithelial cell adhesion molecule selection alone. Owing to high purity (>80%) of the selected circulating tumor cells, molecular analysis of both circulating tumor cell subpopulations was carried out in bulk, including next generation sequencing, mutation analysis, and gene expression. Results suggested fibroblast activation protein alpha and epithelial cell adhesion molecule circulating tumor cells are distinct subpopulations and the use of these in concert can provide information needed to navigate through cancer disease management challenges.en_US
dc.publisherNature Publishing Groupen_US
dc.rightsThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.en_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.titleDiscrete microfluidics for the isolation of circulating tumor cell subpopulations targeting fibroblast activation protein alpha and epithelial cell adhesion moleculeen_US
dc.typeArticleen_US
kusw.kudepartmentChemistryen_US
dc.identifier.doi10.1038/s41698-017-0028-8en_US
kusw.oaversionScholarly/refereed, author accepted manuscripten_US
kusw.oapolicyThis item meets KU Open Access policy criteria.en_US
dc.rights.accessrightsopenAccessen_US


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This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Except where otherwise noted, this item's license is described as: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.