Taxotere suppresses breast cancer growth through inducing lincRNA-p21 expression
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Issue Date
2017-05-31Author
Guo, Yuxiao
Publisher
University of Kansas
Format
27 pages
Type
Thesis
Degree Level
M.A.
Discipline
Molecular Biosciences
Rights
Copyright held by the author.
Metadata
Show full item recordAbstract
It has been reported that long non-coding RNA lincRNA-p21 is induced upon radiation and chemotherapy. This induction contributes to DNA-damage repair, cell death and cell cycle regulation. In this study, we focused on Taxotere (TXT) mediated chemotherapy of breast cancer cells and found that lincRNA-p21 is robotically induced upon TXT treatment. Secondly, we observed increased chemoresistance in three different breast cancer cell lines with lincRNA-p21 knockdown. Mechanistically, we found that lincRNA-p21 knockdown lead to decreased cell death during chemotherapy comparing to the negative control. In addition, our work showed that p21 is a downstream target of lincRNA-p21 in human breast cancer cells, and the loss of lincRNA-p21 caused p21 downregulation at both the RNA and protein levels.
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- Molecular Biosciences Dissertations and Theses [270]
- Theses [3944]
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