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    Evaluation of a Portable Microchip Electrophoresis Fluorescence Detection System for the Analysis of Amino Acid Neurotransmitters in Brain Dialysis Samples

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    Oborny_NBK_2016.pdf (873.5Kb)
    Issue Date
    2016-01-10
    Author
    Oborny, Nathan John
    Melo Costa, Elton E.
    Suntornsuk, Leena
    Abreu, Fabiane
    Lunte, Susan M.
    Publisher
    Nihon Bunseki Kagakkai
    Type
    Article
    Article Version
    Scholarly/refereed, publisher version
    Rights
    © 2016 by The Japan Society for Analytical Chemistry
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    Abstract
    A portable fluorescence detection system for use with microchip electrophoresis was developed and compared to a benchtop system. Using this system, six neuroactive amines commonly found in brain dialysate (arginine, citrulline, taurine, histamine, glutamate, and aspartate) were derivatized offline with naphthalene-2,3-dicarboxaldehyde/cyanide, separated electrophoretically, and detected by fluorescence. The limits of detection for the analytes of interest were 50 – 250 nM for the benchtop system and 250 nM – 1.3 μM for the portable system, both of which were adequate for most analyte detection in brain microdialysis samples. The portable system was then demonstrated for the detection of the same six amines in a rat brain microdialysis sample.
    URI
    http://hdl.handle.net/1808/25328
    DOI
    https://doi.org/10.2116/analsci.32.35
    Collections
    • Bioengineering Program Scholarly Works [152]
    Citation
    Oborny, N. J., Costa, E. E., Suntornsuk, L., Abreu, F. C., & Lunte, S. M. (2016). Evaluation of a Portable Microchip Electrophoresis Fluorescence Detection System for the Analysis of Amino Acid Neurotransmitters in Brain Dialysis Samples. Analytical Sciences, 32(1), 35-40. doi:10.2116/analsci.32.35

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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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