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    Glycoprotein profiling by electrospray mass spectrometry

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    desaire_glycoprotein.pdf (264.3Kb)
    Issue Date
    2004-05
    Author
    Jiang, Hui
    Desaire, Heather
    Butnev, Vladimir Y.
    Bousfield, George R.
    Publisher
    ELSEVIER SCIENCE INC
    Format
    270691 bytes
    Type
    Article
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    Abstract
    This work compares several different methods of site-specific analysis of glycoproteins using electrospray mass spectrometry. The glycoprotein, oLHalpha (ovine luteinizing hormone, a-subunit) was chosen as an appropriate example protein for these studies because of its biological relevance and extreme microheterogeneity. More than 20 unique glycoforms were detected for this glycoprotein at the Asn(56) site of oLHalpha. The carbohydrates present at this site affect receptor binding affinity, so understanding the great variety in the composition of these carbohydrates is important in studying ligand binding interactions. MS data was acquired on a quadrupole ion trap, a triple quadrupole, and a quadrupole time of flight mass spectrometer, and carbohydrate composition at the Asn(56) site of oLHalpha was determined using these instruments. Additionally, neutral loss and precursor ion scanning modes were also used to identify the glycoforms present, and these techniques were compared to the standard MS data. Of the three instruments compared in the study, the qTOF mass spectrometer achieved the lowest sample consumption, but all three instruments were useful in profiling the glycopeptide composition. (C) 2004 American Society for Mass Spectrometry.
    URI
    http://hdl.handle.net/1808/251
    Collections
    • Chemistry Scholarly Works [610]
    Citation
    Jiang, H; Desaire, H; Butnev, VY; Bousfield, GR. Glycoprotein profiling by electrospray mass spectrometry. JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY. May 2004. 15(5):750-758.

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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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