dc.contributor.author | Krause, Mary Elizabeth | |
dc.contributor.author | Glass, Amanda M. | |
dc.contributor.author | Jackson, Timothy A. | |
dc.contributor.author | Laurence, Jennifer S. | |
dc.date.accessioned | 2017-06-13T18:40:06Z | |
dc.date.available | 2017-06-13T18:40:06Z | |
dc.date.issued | 2011-03-21 | |
dc.identifier.citation | Krause, M. E., Glass, A. M., Jackson, T. A., & Laurence, J. S. (2011). MAPping the Chiral Inversion and Structural Transformation of a Metal-Tripeptide Complex having Ni-SOD Activity. Inorganic Chemistry, 50(6), 2479–2487. http://doi.org/10.1021/ic102295s | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/24489 | |
dc.description | This document is the Accepted Manuscript version of a Published Work that appeared in final form in the Inorganic Chemistry, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://doi.org/10.1021/ic102295s. | en_US |
dc.description.abstract | The metal abstraction peptide (MAP) tag is a tripeptide sequence capable of abstracting a metal ion from a chelator and binding it with extremely high affinity at neutral pH. Initial studies on the nickel-bound form of the complex demonstrate that the tripeptide asparagine-cysteine-cysteine (NCC) binds metal with 2N:2S, square planar geometry and behaves as both a structural and functional mimic of Ni superoxide dismutase (Ni-SOD). Electronic absorption, circular dichroism (CD), and magnetic CD (MCD) data collected for Ni-NCC are consistent with a diamagnetic NiII center. It is apparent from the CD signal of Ni-NCC that the optical activity of the complex changes over time. Mass spectrometry data show that the mass of the complex is unchanged. Combined with the CD data, this suggests that chiral rearrangement of the complex occurs. Following incubation of the nickel-containing peptide in D2O and back-exchange into H2O, incorporation of deuterium into non-exchangeable positions is observed, indicating chiral inversion occurs at two of the alpha carbon atoms in the peptide. Control peptides were used to further characterize the chirality of the final nickel-peptide complex, and DFT calculations were performed to validate the hypothesized position of the chiral inversions. In total, these data indicate Ni-SOD activity is increased proportionally to the degree of structural change in the complex over time, as cross-correlation between the change in CD signal and change in SOD activity reveals a linear relationship. | en_US |
dc.publisher | American Chemical Society | en_US |
dc.subject | Chiral inversion | en_US |
dc.subject | Ni-NCC | en_US |
dc.subject | Metal Abstraction Peptide (MAP) | en_US |
dc.subject | D-amino acid | en_US |
dc.subject | D-Cys | en_US |
dc.subject | Square planar | en_US |
dc.subject | Peptide-metal complex | en_US |
dc.subject | Ni-SOD | en_US |
dc.subject | Superoxide dismutase | en_US |
dc.title | MAPping the Chiral Inversion and Structural Transformation of a Metal-Tripeptide Complex having Ni-SOD Activity | en_US |
dc.type | Article | en_US |
kusw.kuauthor | Krause, Mary E. | |
kusw.kuauthor | Glass, Amanda M. | |
kusw.kuauthor | Jackson, Timothy A. | |
kusw.kuauthor | Laurence, Jennifer S. | |
kusw.kudepartment | Chemistry | en_US |
dc.identifier.doi | 10.1021/ic102295s | en_US |
kusw.oaversion | Scholarly/refereed, author accepted manuscript | en_US |
kusw.oapolicy | This item meets KU Open Access policy criteria. | en_US |
dc.identifier.pmid | PMC3059394 | en_US |
dc.rights.accessrights | openAccess | |