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dc.contributor.authorFang, Wei Bin
dc.contributor.authorYao, Min
dc.contributor.authorJokar, Iman
dc.contributor.authorAlhakamy, Nabil A.
dc.contributor.authorBerkland, Cory J.
dc.contributor.authorChen, Jin
dc.contributor.authorBrantley-Sieders, Dana
dc.contributor.authorCheng, Nikki
dc.date.accessioned2017-05-31T20:28:15Z
dc.date.available2017-05-31T20:28:15Z
dc.date.issued2015-04
dc.identifier.citationFang, W.B., Yao, M., Jokar, I. et al. Breast Cancer Res Treat (2015) 150: 309. doi:10.1007/s10549-015-3324-4en_US
dc.identifier.urihttp://hdl.handle.net/1808/24321
dc.description.abstractLuminal A and B breast cancers are the most prevalent forms of breast cancer diagnosed in women. Compared to luminal A breast cancer patients, patients with luminal B breast cancers experience increased disease recurrence and lower overall survival. The mechanisms that regulate the luminal B subtype remain poorly understood. The chemokine CCL2 is overexpressed in breast cancer, correlating with poor patient prognosis. The purpose of this study was to determine the role of CCL2 expression in luminal B breast cancer cells. Breast tissues, MMTV-PyVmT and MMTV-Neu transgenic mammary tumors forming luminal B-like lesions, were immunostained for CCL2 expression. To determine the role of CCL2 in breast cancer cells, CCL2 gene expression was silenced in mammary tumor tissues and cells using TAT cell-penetrating peptides non-covalently cross linked to siRNAs (Ca-TAT/siRNA). CCL2 expression was examined by ELISA and flow cytometry. Cell growth and survival were analyzed by flow cytometry, immunocytochemistry, and fluorescence microscopy. CCL2 expression was significantly increased in luminal B breast tumors, MMTV-PyVmT and MMTV-Neu mammary tumors, compared or normal breast tissue or luminal A breast tumors. Ca-TAT delivery of CCL2 siRNAs significantly reduced CCL2 expression in PyVmT mammary tumors, and decreased cell proliferation and survival. CCL2 gene silencing in PyVmT carcinoma cells or BT474 luminal B breast cancer cells decreased cell growth and viability associated with increased necrosis and autophagy. CCL2 expression is overexpressed in luminal B breast cancer cells and is important for regulating cell growth and survival by inhibiting necrosis and autophagy.en_US
dc.publisherSpringer Verlagen_US
dc.rights© Springer Science + Business Media New York 2015en_US
dc.subjectCCL2en_US
dc.subjectsiRNAen_US
dc.subjectTAT cell-penetrating peptideen_US
dc.subjectLuminal breast canceren_US
dc.subjectNecrosisen_US
dc.subjectAutophagyen_US
dc.titleThe CCL2 chemokine is a negative regulator of autophagy and necrosis in liminal B breast cancer cellsen_US
dc.typeArticleen_US
kusw.kuauthorAlhakamy, Nabil
kusw.kuauthorBerkland, Cory J.
kusw.kudepartmentPharmaceutical Chemistryen_US
dc.identifier.doi10.1007/s10549-015-3324-4en_US
kusw.oaversionScholarly/refereed, author accepted manuscripten_US
kusw.oapolicyThis item meets KU Open Access policy criteria.en_US
dc.identifier.pmidPMC4456035en_US
dc.rights.accessrightsopenAccess


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