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1H, 13C and 15N Backbone Assignment of the EC-1 Domain of Human E-Cadherin
dc.contributor.author | Prasasty, Vivitri D. | |
dc.contributor.author | Krause, Mary Elizabeth | |
dc.contributor.author | Tambunan, Usman S. F. | |
dc.contributor.author | Anbanandam, Asokan | |
dc.contributor.author | Laurence, Jennifer S. | |
dc.contributor.author | Siahaan, Teruna J. | |
dc.date.accessioned | 2017-05-10T17:29:49Z | |
dc.date.available | 2017-05-10T17:29:49Z | |
dc.date.issued | 2014-02-08 | |
dc.identifier.citation | Prasasty, V. D., Krause, M. E., Tambunan, U. S. F., Anbanandam, A., Laurence, J. S., & Siahaan, T. J. (2015). 1H, 13C and 15N Backbone Assignment of the EC-1 Domain of Human E-Cadherin. Biomolecular NMR Assignments, 9(1), 31–35. http://doi.org/10.1007/s12104-013-9539-6 | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/24071 | |
dc.description | The final publication is available at Springer via http://dx.doi.org/10.1007/s12104-013-9539-6 | en_US |
dc.description.abstract | The EC1 domain of E-cadherin has been shown to be important for cadherin-cadherin homophilic interactions. Cadherins are responsible for calcium-mediated cell-cell adhesion located at the adherens junction of the biological barriers (i.e., intestinal mucosa and the blood-brain barrier (BBB). Cadherin peptides can modulate cadherin interactions to improve drug delivery through the blood-brain barriers (BBB). However, the mechanism of modulating the E-cadherin interactions by cadherin peptides has not been fully elucidated. To provide a basis for subsequent examination of the structure and peptide-binding properties of the EC1 domain of human E-cadherin using solution NMR spectroscopy, the 1H, 13C and 15N backbone resonance of the uniformly labeled-EC1 were assigned and the secondary structure was determined based on the chemical shift values. These resonance assignments are essential for assessing protein-ligand interactions and are reported here. | en_US |
dc.publisher | Springer Verlag | en_US |
dc.rights | © Springer Science+Business Media Dordrecht 2014 | en_US |
dc.subject | Human E-cadherin | en_US |
dc.subject | EC1 | en_US |
dc.subject | Backbone resonance assignment | en_US |
dc.subject | Multidimensional NMR | en_US |
dc.title | 1H, 13C and 15N Backbone Assignment of the EC-1 Domain of Human E-Cadherin | en_US |
dc.type | Article | en_US |
kusw.kuauthor | Prasasty, Vivitri D. | |
kusw.kuauthor | Krause, Mary E. | |
kusw.kuauthor | Laurence, Jennifer S. | |
kusw.kuauthor | Siahaan, Teruna J. | |
kusw.kudepartment | Pharmaceutical Chemistry | en_US |
dc.identifier.doi | 10.1007/s12104-013-9539-6 | en_US |
kusw.oaversion | Scholarly/refereed, author accepted manuscript | en_US |
kusw.oapolicy | This item meets KU Open Access policy criteria. | en_US |
dc.identifier.pmid | PMC4133310 | en_US |
dc.rights.accessrights | openAccess |