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dc.contributor.authorWilliams, S. Janette
dc.contributor.authorWang, Qun
dc.contributor.authorMacGregor, Ronal R.
dc.contributor.authorSiahaan, Teruna J.
dc.contributor.authorStehno-Bittel, Lisa
dc.contributor.authorBerkland, Cory J.
dc.date.accessioned2017-05-09T18:12:05Z
dc.date.available2017-05-09T18:12:05Z
dc.date.issued2009-08
dc.identifier.citationWilliams, S. J., Wang, Q., MacGregor, R. R., Siahaan, T. J., Stehno-Bittel, L., & Berkland, C. (2009). Adhesion of Pancreatic Beta Cells to Biopolymer Films. Biopolymers, 91(8), 676–685. http://doi.org/10.1002/bip.21196en_US
dc.identifier.urihttp://hdl.handle.net/1808/24046
dc.descriptionThis is the peer reviewed version of the following article: Williams, S. J., Wang, Q., MacGregor, R. R., Siahaan, T. J., Stehno-Bittel, L., & Berkland, C. (2009). Adhesion of Pancreatic Beta Cells to Biopolymer Films. Biopolymers, 91(8), 676–685. http://doi.org/10.1002/bip.21196, which has been published in final form at doi.org/10.1002/bip.21196. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archivingen_US
dc.description.abstractDramatic reversal of Type 1 diabetes in patients receiving pancreatic islet transplants continues to prompt vigorous research concerning the basic mechanisms underlying patient turnaround. At the most fundamental level, transplanted islets must maintain viability and function in vitro and in vivo and should be protected from host immune rejection. Our previous reports showed enhancement of islet viability and insulin secretion per tissue mass for small islets (<125 µm) as compared to large islets (>125 µm), thus, demonstrating the effect of enhancing the mass transport of islets (i.e. increasing tissue surface area to volume ratio). Here, we report the facile dispersion of rat islets into individual cells that are layered onto the surface of a biopolymer film towards the ultimate goal of improving mass transport in islet tissue. The tightly packed structure of intact islets was disrupted by incubating in calcium-free media resulting in fragmented islets, which were further dispersed into individual or small groups of cells by using a low concentration of papain. The dispersed cells were screened for adhesion to a range of biopolymers and the nature of cell adhesion was characterized for selected groups by quantifying adherent cells, measuring the surface area coverage of the cells, and immunolabeling cells for adhesion proteins interacting with selected biopolymers. Finally, beta cells in suspension were centrifuged to form controlled numbers of cell layers on films for future work determining the mass transport limitations in the adhered tissue constructs.en_US
dc.publisherWileyen_US
dc.rights© 2009 Wiley Periodicals, Inc.en_US
dc.subjectIsleten_US
dc.subjectBeta cellen_US
dc.subjectThin filmen_US
dc.subjectBiopolymersen_US
dc.subjectCell adhesionen_US
dc.titleAdhesion of Pancreatic Beta Cells to Biopolymer Filmsen_US
dc.typeArticleen_US
kusw.kuauthorWang, Qun
kusw.kuauthorBerkland, Cory J.
kusw.kuauthorSiahaan, Teruna J.
kusw.kudepartmentChemical and Petroleum Engineeringen_US
kusw.kudepartmentPharmaceutical Chemistryen_US
dc.identifier.doi10.1002/bip.21196en_US
dc.identifier.orcidhttps://orcid.org/0000-0002-8879-6124
kusw.oaversionScholarly/refereed, author accepted manuscripten_US
kusw.oapolicyThis item meets KU Open Access policy criteria.en_US
dc.identifier.pmidPMC2778605en_US
dc.rights.accessrightsopenAccess


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