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dc.contributor.authorArmendariz, Kevin P.
dc.contributor.authorDunn, Robert C.
dc.identifier.citationArmendariz, K. P., & Dunn, R. C. (2013). Ganglioside Influence on Phospholipid Films Investigated with Single Molecule Fluorescence Measurements. The Journal of Physical Chemistry. B, 117(26), 7959–7966.
dc.description.abstractSingle molecule fluorescence measurements are used to probe the effects of GM1 in DPPC monolayers. Langmuir-Blodgett films of GM1 and DPPC were doped with ~10−8 mol% of the fluorescent lipid probe, BODIPY-PC, and transferred onto glass substrates at 23 mN/m. As shown previously, the individual orientation of each BODIPY-PC probe in the membrane can be measured using defocused polarized total internal reflection fluorescence microscopy, revealing changes in film properties at the molecular level. Here, BODIPY-PC tilt angle histograms are used to characterize the effects of GM1 in DPPC films from 0.05 mol% to 100 mol% GM1. At high GM1 levels (>5 mol% GM1), trends in the single molecule measurements agree with previous bulk measurements showing the turnover from condensing to expanding influence of GM1 at ~20 mol%, thus validating the single molecule approach. At biologically relevant, low concentrations of GM1 (<5 mol% GM1), where bulk fluorescence measurements are less informative, the single molecule measurements reveal a marked influence of GM1 on film properties. The addition of trace amounts of GM1 to DPPC films leads to an expansion of the film which continues to 0.10 mol% GM1, above which the trend reverses and the condensing effect previously noted is observed.en_US
dc.publisherAmerican Chemical Societyen_US
dc.rightsThis document is the Accepted Manuscript version of a Published Work that appeared in final form in the Journal of Physical Chemistry B, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see
dc.subjectLangmuir-Blodgett filmsen_US
dc.subjectTotal internal reflectionen_US
dc.titleGanglioside Influence on Phospholipid Films Investigated with Single Molecule Fluorescence Measurementsen_US
kusw.kuauthorArmendariz, Kevin P.
kusw.kuauthorDunn, Robert C.
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kusw.oaversionScholarly/refereed, author accepted manuscripten_US
kusw.oapolicyThis item meets KU Open Access policy criteria.en_US

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