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Ganglioside Influence on Phospholipid Films Investigated with Single Molecule Fluorescence Measurements
dc.contributor.author | Armendariz, Kevin P. | |
dc.contributor.author | Dunn, Robert C. | |
dc.date.accessioned | 2017-04-27T19:42:28Z | |
dc.date.available | 2017-04-27T19:42:28Z | |
dc.date.issued | 2013-07-03 | |
dc.identifier.citation | Armendariz, K. P., & Dunn, R. C. (2013). Ganglioside Influence on Phospholipid Films Investigated with Single Molecule Fluorescence Measurements. The Journal of Physical Chemistry. B, 117(26), 7959–7966. http://doi.org/10.1021/jp405312a | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/23850 | |
dc.description.abstract | Single molecule fluorescence measurements are used to probe the effects of GM1 in DPPC monolayers. Langmuir-Blodgett films of GM1 and DPPC were doped with ~10−8 mol% of the fluorescent lipid probe, BODIPY-PC, and transferred onto glass substrates at 23 mN/m. As shown previously, the individual orientation of each BODIPY-PC probe in the membrane can be measured using defocused polarized total internal reflection fluorescence microscopy, revealing changes in film properties at the molecular level. Here, BODIPY-PC tilt angle histograms are used to characterize the effects of GM1 in DPPC films from 0.05 mol% to 100 mol% GM1. At high GM1 levels (>5 mol% GM1), trends in the single molecule measurements agree with previous bulk measurements showing the turnover from condensing to expanding influence of GM1 at ~20 mol%, thus validating the single molecule approach. At biologically relevant, low concentrations of GM1 (<5 mol% GM1), where bulk fluorescence measurements are less informative, the single molecule measurements reveal a marked influence of GM1 on film properties. The addition of trace amounts of GM1 to DPPC films leads to an expansion of the film which continues to 0.10 mol% GM1, above which the trend reverses and the condensing effect previously noted is observed. | en_US |
dc.publisher | American Chemical Society | en_US |
dc.rights | This document is the Accepted Manuscript version of a Published Work that appeared in final form in the Journal of Physical Chemistry B, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://dx.doi.org/10.1021/jp405312a. | en_US |
dc.subject | GM1 | en_US |
dc.subject | DPPC | en_US |
dc.subject | Fluorescence | en_US |
dc.subject | Partitioning | en_US |
dc.subject | Langmuir-Blodgett films | en_US |
dc.subject | Total internal reflection | en_US |
dc.title | Ganglioside Influence on Phospholipid Films Investigated with Single Molecule Fluorescence Measurements | en_US |
dc.type | Article | en_US |
kusw.kuauthor | Armendariz, Kevin P. | |
kusw.kuauthor | Dunn, Robert C. | |
kusw.kudepartment | Chemistry | en_US |
dc.identifier.doi | 10.1021/jp405312a | en_US |
kusw.oaversion | Scholarly/refereed, author accepted manuscript | en_US |
kusw.oapolicy | This item meets KU Open Access policy criteria. | en_US |
dc.identifier.pmid | PMC3756589 | en_US |
dc.rights.accessrights | openAccess |