Classic Maximum Entropy Recovery of the Average Joint Distribution of Apparent FRET Efficiency and Fluorescence Photons for Single-molecule Burst Measurements

DeVore, Matthew S.; Gull, Stephen F.; Johnson, Carey K.

dc.contributor.author	DeVore, Matthew S.
dc.contributor.author	Gull, Stephen F.
dc.contributor.author	Johnson, Carey K.
dc.date.accessioned	2017-04-27T18:13:45Z
dc.date.available	2017-04-27T18:13:45Z
dc.date.issued	2012-04-05
dc.identifier.citation	DeVore, M. S., Gull, S. F., & Johnson, C. K. (2012). Classic Maximum Entropy Recovery of the Average Joint Distribution of Apparent FRET Efficiency and Fluorescence Photons for Single-molecule Burst Measurements. The Journal of Physical Chemistry. B, 116(13), 4006–4015. http://doi.org/10.1021/jp209861u	en_US
dc.identifier.uri	http://hdl.handle.net/1808/23839
dc.description.abstract	We describe a method for analysis of single-molecule Förster resonance energy transfer (FRET) burst measurements using classic maximum entropy. Classic maximum entropy determines the Bayesian inference for the joint probability describing the total fluorescence photons and the apparent FRET efficiency. The method was tested with simulated data and then with DNA labeled with fluorescent dyes. The most probable joint distribution can be marginalized to obtain both the overall distribution of fluorescence photons and the apparent FRET efficiency distribution. This method proves to be ideal for determining the distance distribution of FRET-labeled biomolecules, and it successfully predicts the shape of the recovered distributions.	en_US
dc.publisher	American Chemical Society	en_US
dc.rights	This document is the Accepted Manuscript version of a Published Work that appeared in final form in the Journal of Physical Chemistry B, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://dx.doi.org/10.1021/jp209861u.	en_US
dc.subject	Single-molecule FRET	en_US
dc.subject	Bayesian analysis	en_US
dc.subject	Photon distribution analysis	en_US
dc.subject	Maximum entropy	en_US
dc.title	Classic Maximum Entropy Recovery of the Average Joint Distribution of Apparent FRET Efficiency and Fluorescence Photons for Single-molecule Burst Measurements	en_US
dc.type	Article	en_US
kusw.kuauthor	DeVore, Matthew S.
kusw.kuauthor	Johnson, Carey K.
kusw.kudepartment	Chemistry	en_US
dc.identifier.doi	10.1021/jp209861u	en_US
kusw.oaversion	Scholarly/refereed, author accepted manuscript	en_US
kusw.oapolicy	This item meets KU Open Access policy criteria.	en_US
dc.identifier.pmid	PMC3320690	en_US
dc.rights.accessrights	openAccess

Files in this item

Name:: DeVore_2012.pdf
Size:: 1.616Mb
Format:: PDF

View/Open

This item appears in the following Collection(s)

The University of Kansas prohibits discrimination on the basis of race, color, ethnicity, religion, sex, national origin, age, ancestry, disability, status as a veteran, sexual orientation, marital status, parental status, gender identity, gender expression and genetic information in the University’s programs and activities. The following person has been designated to handle inquiries regarding the non-discrimination policies: Director of the Office of Institutional Opportunity and Access, IOA@ku.edu, 1246 W. Campus Road, Room 153A, Lawrence, KS, 66045, (785)864-6414, 711 TTY.