Glassy-State Stabilization of a Dominant Negative Inhibitor Anthrax Vaccine Containing Aluminum Hydroxide and Glycopyranoside Lipid A Adjuvants
dc.contributor.author | Hassett, Kimberly J. | |
dc.contributor.author | Vance, David J. | |
dc.contributor.author | Jain, Nishant K. | |
dc.contributor.author | Shani, Neha | |
dc.contributor.author | Rabia, Lilia A. | |
dc.contributor.author | Cousins, Megan C. | |
dc.contributor.author | Joshi, Sangeeta B. | |
dc.contributor.author | Volkin, David B. | |
dc.contributor.author | Middaugh, C. Russell | |
dc.contributor.author | Mantis, Nicholas J. | |
dc.contributor.author | Carpenter, John F. | |
dc.contributor.author | Randolph, Theodore W. | |
dc.date.accessioned | 2017-04-25T18:22:10Z | |
dc.date.available | 2017-04-25T18:22:10Z | |
dc.date.issued | 2015-02 | |
dc.identifier.citation | Hassett, K. J., Vance, D. J., Jain, N. K., Sahni, N., Rabia, L. A., Cousins, M. C., … Randolph, T. W. (2015). Glassy-State Stabilization of a Dominant Negative Inhibitor Anthrax Vaccine Containing Aluminum Hydroxide and Glycopyranoside Lipid A Adjuvants. Journal of Pharmaceutical Sciences, 104(2), 627–639. http://doi.org/10.1002/jps.24295 | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/23768 | |
dc.description.abstract | During transport and storage, vaccines may be exposed to temperatures outside of the range recommended for storage, potentially causing efficacy losses. To better understand and prevent such losses, Dominant Negative Inhibitor (DNI), a recombinant protein antigen for a candidate vaccine against anthrax, was formulated as a liquid and as a glassy lyophilized powder with the adjuvants aluminum hydroxide and glycopyranoside lipid A (GLA). Freeze-thawing of the liquid vaccine caused the adjuvants to aggregate and decreased its immunogenicity in mice. Immunogenicity of liquid vaccines also decreased when stored at 40 °C for 8 weeks, as measured by decreases in neutralizing antibody titers in vaccinated mice. Concomitant with efficacy losses at elevated temperatures, changes in DNI structure were detected by fluorescence spectroscopy and increased deamidation was observed by capillary isoelectric focusing (cIEF) after only 1 week of storage of the liquid formulation at 40 °C. In contrast, upon lyophilization, no additional deamidation after 4 weeks at 40 °C and no detectable changes in DNI structure or reduction in immunogenicity after 16 weeks at 40 °C was observed. Vaccines containing aluminum hydroxide and GLA elicited higher immune responses than vaccines adjuvanted with only aluminum hydroxide, with more mice responding to a single dose. | en_US |
dc.publisher | Elsevier | en_US |
dc.rights | This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License 4.0 (CC BY-NC-ND 4.0), which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | en_US |
dc.subject | Lyophilization | en_US |
dc.subject | Anthrax | en_US |
dc.subject | Dominant Negative Inhibitor | en_US |
dc.subject | Vaccine Adjuvants | en_US |
dc.subject | Aluminum | en_US |
dc.subject | Glycopyranoside Lipid A (GLA) | en_US |
dc.subject | Vaccines | en_US |
dc.subject | Freeze-thaw | en_US |
dc.subject | Stability | en_US |
dc.subject | Formulation | en_US |
dc.title | Glassy-State Stabilization of a Dominant Negative Inhibitor Anthrax Vaccine Containing Aluminum Hydroxide and Glycopyranoside Lipid A Adjuvants | en_US |
dc.type | Article | en_US |
kusw.kuauthor | Jain, Nishant K. | |
kusw.kuauthor | Sahni, Neha | |
kusw.kuauthor | Joshi, Sangeeta B. | |
kusw.kuauthor | Volkin, David B. | |
kusw.kuauthor | Middaugh, C. Russell | |
kusw.kudepartment | Pharmaceutical Chemistry | en_US |
dc.identifier.doi | 10.1002/jps.24295 | en_US |
kusw.oaversion | Scholarly/refereed, author accepted manuscript | en_US |
kusw.oapolicy | This item meets KU Open Access policy criteria. | en_US |
dc.identifier.pmid | PMC4312196 | en_US |
dc.rights.accessrights | openAccess |
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Except where otherwise noted, this item's license is described as: This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License 4.0 (CC BY-NC-ND 4.0), which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.