Controlling Ligand Surface Density Optimizes Nanoparticle Binding to ICAM-1
dc.contributor.author | Fakhari, Amir | |
dc.contributor.author | Baoum, Abdulgader Ahmed | |
dc.contributor.author | Le, Khoi Ba | |
dc.contributor.author | Siahaan, Teruna J. | |
dc.contributor.author | Berkland, Cory J. | |
dc.date.accessioned | 2017-04-19T19:03:54Z | |
dc.date.available | 2017-04-19T19:03:54Z | |
dc.date.issued | 2011-03 | |
dc.identifier.citation | Fakhari, A., Baoum, A., Le, K. B., Siahaan, T. J., & Berkland, C. (2011). Controlling Ligand Surface Density Optimizes Nanoparticle Binding to ICAM-1. Journal of Pharmaceutical Sciences, 100(3), 1045–1056. http://doi.org/10.1002/jps.22342 | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/23741 | |
dc.description.abstract | During infection, pathogens utilize surface receptors to gain entry into intracellular compartments. Multiple receptor-ligand interactions that lead to pathogen internalization have been identified and the importance of multivalent ligand binding as a means to facilitate internalization has emerged. The effect of ligand density, however; is less well known. In this study, ligand density was examined using poly(DL-lactic-co-glycolic acid) nanoparticles (PLGA NPs). A cyclic peptide, cLABL, was used as a targeting moiety as it is a known ligand for intercellular cell adhesion molecule-1 (ICAM-1). To modulate the number of reactive sites on the surface of PLGA NPs, modified Pluronic® with carboxyl groups and Pluronic® with hydroxyl groups were combined at different ratios and the particle properties were examined. Utilizing a surfactant mixture directly affected the particle charge and the number of reactive sites for cLABL conjugation. The surface density of cLABL peptide increased as the relative amount of reactive Pluronic® was increased. Studies using carcinomic human alveolar basal epithelial cells (A549) showed that cLABL density may be optimized to improve cellular uptake. These results compliment other studies suggesting surface density of the targeting moiety on the NP surface should be considered to enhance the effect of ligands employed for cell targeting. | en_US |
dc.publisher | Elsevier | en_US |
dc.rights | This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License 4.0 (CC BY-NC-ND 4.0), which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | en_US |
dc.subject | Ligand density | en_US |
dc.subject | Poly(DL-lactic-co-glycolic acid) | en_US |
dc.subject | Nanoparticles | en_US |
dc.subject | cLABL | en_US |
dc.subject | Pluronic® | en_US |
dc.subject | Binding and cellular uptake | en_US |
dc.title | Controlling Ligand Surface Density Optimizes Nanoparticle Binding to ICAM-1 | en_US |
dc.type | Article | en_US |
kusw.kuauthor | Fakhari, Amir | |
kusw.kuauthor | Baoum, Abdulgader Ahmed | |
kusw.kuauthor | Le, Khoi Ba | |
kusw.kuauthor | Siahaan, Teruna J. | |
kusw.kuauthor | Berkland, Cory J. | |
kusw.kudepartment | Pharmaceutical Chemistry | en_US |
kusw.kudepartment | Chemical and Petroleum Engineering | en_US |
dc.identifier.doi | 10.1002/jps.22342 | en_US |
kusw.oaversion | Scholarly/refereed, author accepted manuscript | en_US |
kusw.oapolicy | This item meets KU Open Access policy criteria. | en_US |
dc.rights.accessrights | openAccess |
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Except where otherwise noted, this item's license is described as: This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License 4.0 (CC BY-NC-ND 4.0), which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.