Calcium condensation of DNA complexed with cell-penetrating peptides offers efficient, noncytotoxic gene delivery
dc.contributor.author | Baoum, Abdulgader Ahmed | |
dc.contributor.author | Berkland, Cory J. | |
dc.date.accessioned | 2017-04-19T17:47:02Z | |
dc.date.available | 2017-04-19T17:47:02Z | |
dc.date.issued | 2011-05 | |
dc.identifier.citation | Baoum, A. A., & Berkland, C. J. (2011). Calcium condensation of DNA complexed with cell-penetrating peptides offers efficient, noncytotoxic gene delivery. Journal of Pharmaceutical Sciences, 100(5), 1637–1642. http://doi.org/10.1002/jps.22407 | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/23737 | |
dc.description.abstract | Drug delivery strategies using cell penetrating peptides (CPPs) have been widely explored to improve the intracellular delivery of a large number of cargo molecules. Electrostatic complexation of pDNA using CPPs has been less explored due to the relatively large complexes formed and the low levels of gene expression achieved when using these low molecular weight polycations as DNA condensing agents. Here, condensing nascent CPP polyplexes using CaCl2 produced small and stable nanoparticles leading to gene expression levels higher than observed for control PEI gene vectors. This simple formulation approach showed negligible cytotoxicity in A549 lung epithelial cells and maintained particle size and transfection efficiency even in the presence of serum. | en_US |
dc.publisher | Elsevier | en_US |
dc.rights | This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License 4.0 (CC BY-NC-ND 4.0), which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | en_US |
dc.subject | Gene Delivery | en_US |
dc.subject | Plasmid DNA | en_US |
dc.subject | Cell-penetrating peptides | en_US |
dc.subject | A549 cells | en_US |
dc.title | Calcium condensation of DNA complexed with cell-penetrating peptides offers efficient, noncytotoxic gene delivery | en_US |
dc.type | Article | en_US |
kusw.kuauthor | Baoum, Abdulgader Ahmed | |
kusw.kuauthor | Berkland, Cory J. | |
kusw.kudepartment | Pharmaceutical Chemistry | en_US |
dc.identifier.doi | 10.1002/jps.22407 | en_US |
kusw.oaversion | Scholarly/refereed, author accepted manuscript | en_US |
kusw.oapolicy | This item meets KU Open Access policy criteria. | en_US |
dc.rights.accessrights | openAccess |
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Except where otherwise noted, this item's license is described as: This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License 4.0 (CC BY-NC-ND 4.0), which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.