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Separation and Detection of Peroxynitrite Using Microchip Electrophoresis with Amperometric Detection
dc.contributor.author | Hulvey, Matthew K. | |
dc.contributor.author | Frankenfeld, Celeste | |
dc.contributor.author | Lunte, Susan M. | |
dc.date.accessioned | 2017-04-06T17:08:38Z | |
dc.date.available | 2017-04-06T17:08:38Z | |
dc.date.issued | 2010-03-01 | |
dc.identifier.citation | Hulvey, M. K., Frankenfeld, C. N., & Lunte, S. M. (2010). Separation and Detection of Peroxynitrite Using Microchip Electrophoresis with Amperometric Detection. Analytical Chemistry, 82(5), 1608–1611. http://doi.org/10.1021/ac902821v | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/23593 | |
dc.description.abstract | Peroxynitrite (ONOO-) is a highly reactive species implicated in the pathology of several cardiovascular and neurodegenerative diseases. It is generated in vivo by the diffusion-limited reaction of nitric oxide (NO•) and superoxide anion (•O2-) and is known to be produced during periods of inflammation. Detection of ONOO- is made difficult by its short half-life under physiological conditions (∼1 s). Here we report a method for the separation and detection of ONOOfrom other electroactive species utilizing a microchip electrophoresis device incorporating an amperometric detection scheme. Microchip electrophoresis permits shorter separation times (∼25 s for ONOO-) and higher temporal resolution than conventional capillary electrophoresis (several minutes). This faster analysis allows ONOO- to be detected before substantial degradation occurs, and the increased temporal resolution permits more accurate tracking of dynamic changes in chemical systems. | en_US |
dc.publisher | American Chemical Society | en_US |
dc.rights | Copyright © 2010 American Chemical Society | en_US |
dc.title | Separation and Detection of Peroxynitrite Using Microchip Electrophoresis with Amperometric Detection | en_US |
dc.type | Article | en_US |
kusw.kuauthor | Frankenfeld, Celeste | |
kusw.kuauthor | Lunte, Susan M. | |
kusw.kudepartment | Pharmaceutical Chemistry | en_US |
dc.identifier.doi | 10.1021/ac902821v | en_US |
kusw.oaversion | Scholarly/refereed, author accepted manuscript | en_US |
kusw.oapolicy | This item meets KU Open Access policy criteria. | en_US |
dc.rights.accessrights | openAccess |