ATTENTION: The software behind KU ScholarWorks is being upgraded to a new version. Starting July 15th, users will not be able to log in to the system, add items, nor make any changes until the new version is in place at the end of July. Searching for articles and opening files will continue to work while the system is being updated.
If you have any questions, please contact Marianne Reed at mreed@ku.edu .
A general protease digestion procedure for optimal protein sequence coverage and PTM analysis of recombinant glycoproteins: Application to the characterization of hLOXL2 glycosylation
dc.contributor.author | Rebecchi, Kathryn | |
dc.contributor.author | Go, Eden P. | |
dc.contributor.author | Xu, Li | |
dc.contributor.author | Woodin, Carrie L. | |
dc.contributor.author | Mure, Minae | |
dc.contributor.author | Desaire, Heather | |
dc.date.accessioned | 2017-03-31T20:47:07Z | |
dc.date.available | 2017-03-31T20:47:07Z | |
dc.date.issued | 2011-10-27 | |
dc.identifier.citation | Rebecchi, K. R., Go, E. P., Xu, L., Woodin, C. L., Mure, M., & Desaire, H. (2011). A general protease digestion procedure for optimal protein sequence coverage and PTM analysis of recombinant glycoproteins: Application to the characterization of hLOXL2 glycosylation. Analytical Chemistry, 83(22), 8484–8491. http://doi.org/10.1021/ac2017037 | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/23561 | |
dc.description.abstract | Using recombinant DNA technology for expression of protein therapeutics is a maturing field of pharmaceutical research and development. As recombinant proteins are increasingly utilized as biotherapeutics, improved methodologies ensuring the characterization of post-translational modifications (PTMs) are needed. Typically, proteins prepared for PTM analysis are proteolytically digested and analyzed by mass spectrometry. To assure full coverage of the PTMs on a given protein, one must obtain complete sequence coverage of the protein, which is often quite challenging. The objective of the research described here is to design a protocol that maximizes protein sequence coverage and enables detection of post-translational modifications, specifically N-linked glycosylation. To achieve this objective, a highly efficient proteolytic digest protocol using trypsin was designed by comparing the relative merits of denaturing agents (urea and Rapigest™ SF), reducing agents (dithiothreitol, DTT, and tris(2-carboxyethyl)phophine, TCEP), and various concentrations of alkylating agent (iodoacetamide, IAM). After analysis of human apo-transferrin using various protease digestion protocols, ideal conditions were determined to contain 6 M urea for denaturation, 5 mM TCEP for reduction, 10 mM IAM for alkylation, and 10 mM DTT, to quench excess IAM before the addition of trypsin. This method was successfully applied to a novel recombinant protein, human lysyl oxidase-like 2 (hLOXL2). Furthermore, the glycosylation PTMs were readily detected at two glycosylation sites in the protein. These digestion conditions were specifically designed for PTM analysis of recombinant proteins and biotherapeutics, and the work described herein fills an unmet need in the growing field of biopharmaceutical analysis. | en_US |
dc.publisher | American Chemistry Society | en_US |
dc.rights | © 2011 American Chemical Society | en_US |
dc.title | A general protease digestion procedure for optimal protein sequence coverage and PTM analysis of recombinant glycoproteins: Application to the characterization of hLOXL2 glycosylation | en_US |
dc.type | Article | en_US |
kusw.kuauthor | Rebecchi, Kathryn | |
kusw.kuauthor | Go, Eden | |
kusw.kuauthor | Woodin, Carrie | |
kusw.kuauthor | Mure, Minae | |
kusw.kuauthor | Desaire, Heather | |
kusw.kudepartment | Chemistry | en_US |
dc.identifier.doi | 10.1021/ac2017037 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0003-0309-6127 | |
kusw.oaversion | Scholarly/refereed, author accepted manuscript | en_US |
kusw.oapolicy | This item meets KU Open Access policy criteria. | en_US |
dc.rights.accessrights | openAccess |