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dc.contributor.authorRebecchi, Kathryn
dc.contributor.authorGo, Eden P.
dc.contributor.authorXu, Li
dc.contributor.authorWoodin, Carrie L.
dc.contributor.authorMure, Minae
dc.contributor.authorDesaire, Heather
dc.date.accessioned2017-03-31T20:47:07Z
dc.date.available2017-03-31T20:47:07Z
dc.date.issued2011-10-27
dc.identifier.citationRebecchi, K. R., Go, E. P., Xu, L., Woodin, C. L., Mure, M., & Desaire, H. (2011). A general protease digestion procedure for optimal protein sequence coverage and PTM analysis of recombinant glycoproteins: Application to the characterization of hLOXL2 glycosylation. Analytical Chemistry, 83(22), 8484–8491. http://doi.org/10.1021/ac2017037en_US
dc.identifier.urihttp://hdl.handle.net/1808/23561
dc.description.abstractUsing recombinant DNA technology for expression of protein therapeutics is a maturing field of pharmaceutical research and development. As recombinant proteins are increasingly utilized as biotherapeutics, improved methodologies ensuring the characterization of post-translational modifications (PTMs) are needed. Typically, proteins prepared for PTM analysis are proteolytically digested and analyzed by mass spectrometry. To assure full coverage of the PTMs on a given protein, one must obtain complete sequence coverage of the protein, which is often quite challenging. The objective of the research described here is to design a protocol that maximizes protein sequence coverage and enables detection of post-translational modifications, specifically N-linked glycosylation. To achieve this objective, a highly efficient proteolytic digest protocol using trypsin was designed by comparing the relative merits of denaturing agents (urea and Rapigest™ SF), reducing agents (dithiothreitol, DTT, and tris(2-carboxyethyl)phophine, TCEP), and various concentrations of alkylating agent (iodoacetamide, IAM). After analysis of human apo-transferrin using various protease digestion protocols, ideal conditions were determined to contain 6 M urea for denaturation, 5 mM TCEP for reduction, 10 mM IAM for alkylation, and 10 mM DTT, to quench excess IAM before the addition of trypsin. This method was successfully applied to a novel recombinant protein, human lysyl oxidase-like 2 (hLOXL2). Furthermore, the glycosylation PTMs were readily detected at two glycosylation sites in the protein. These digestion conditions were specifically designed for PTM analysis of recombinant proteins and biotherapeutics, and the work described herein fills an unmet need in the growing field of biopharmaceutical analysis.en_US
dc.publisherAmerican Chemistry Societyen_US
dc.rights© 2011 American Chemical Societyen_US
dc.titleA general protease digestion procedure for optimal protein sequence coverage and PTM analysis of recombinant glycoproteins: Application to the characterization of hLOXL2 glycosylationen_US
dc.typeArticleen_US
kusw.kuauthorRebecchi, Kathryn
kusw.kuauthorGo, Eden
kusw.kuauthorWoodin, Carrie
kusw.kuauthorMure, Minae
kusw.kuauthorDesaire, Heather
kusw.kudepartmentChemistryen_US
dc.identifier.doi10.1021/ac2017037en_US
dc.identifier.orcidhttps://orcid.org/0000-0003-0309-6127
kusw.oaversionScholarly/refereed, author accepted manuscripten_US
kusw.oapolicyThis item meets KU Open Access policy criteria.en_US
dc.rights.accessrightsopenAccess


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