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    Optimization of the Separation of NDA-Derivatized Methylarginines by Capillary and Microchip Electrophoresis

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    Issue Date
    2012-02-17
    Author
    Linz, Thomas H.
    Snyder, Christa M.
    Lunte, Susan M.
    Publisher
    SAGE Publications
    Type
    Article
    Article Version
    Scholarly/refereed, author accepted manuscript
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    Abstract
    The methylated arginines (MAs) monomethylarginine (MMA), asymmetric dimethylarginine (ADMA), and symmetric dimethylarginine (SDMA) have been shown to be independent predictors of cardiovascular disease. This article describes progress regarding the development of an analytical method capable of rapidly analyzing MAs using capillary electrophoresis (CE) and microchip electrophoresis (MCE) with laser-induced fluorescence (LIF) detection. Several parameters including buffer composition and separation voltage were optimized to achieve an ideal separation. The analytes of interest were derivatized with naphthalene-2,3-dicarboxaldehyde (NDA) to produce fluorescent 1-cyanobenz[f]isoindole (CBI) derivatives and then subjected to CE analysis. Baseline resolution of SDMA, ADMA, MMA, and arginine was achieved in less than 8 min. The limits of detection for SDMA, ADMA, MMA, and arginine were determined to be 15, 20, 25, and 5 nM, respectively, which are well below the expected plasma concentrations. The CE separation method was then transferred to a glass MCE device with LIF detection. MAs were baseline resolved in 3 min on-chip using a 14 cm separation channel with detection limits of approximately 10 nM for each species. To the best of the authors’ knowledge, this is the first report of the separation of MAs by MCE.
    URI
    http://hdl.handle.net/1808/23559
    DOI
    https://doi.org/10.1177/2211068211424551
    Collections
    • Chemistry Scholarly Works [610]
    Citation
    Linz, Thomas H., Christa M. Snyder, and Susan M. Lunte. "Optimization of the Separation of NDA-Derivatized Methylarginines by Capillary and Microchip Electrophoresis." Journal of Laboratory Automation 17.1 (2012): 24-31.

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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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