ICAM-1 Targeting of Doxorubicin-Loaded PLGA Nanoparticles to Lung Epithelial Cells
dc.contributor.author | Chittasupho, Chuda | |
dc.contributor.author | Xie, Sheng-Xue | |
dc.contributor.author | Baoum, Abdulgader Ahmed | |
dc.contributor.author | Yakovleva, Tatyana | |
dc.contributor.author | Siahaan, Teruna J. | |
dc.contributor.author | Berkland, Cory J. | |
dc.date.accessioned | 2017-03-08T20:22:45Z | |
dc.date.available | 2017-03-08T20:22:45Z | |
dc.date.issued | 2010-05-12 | |
dc.identifier.citation | Chittasupho, Chuda, Sheng-Xue Xie, Abdulgader Baoum, Tatyana Yakovleva, Teruna J. Siahaan, and Cory J. Berkland. "ICAM-1 Targeting of Doxorubicin-loaded PLGA Nanoparticles to Lung Epithelial Cells." European Journal of Pharmaceutical Sciences 37.2 (2009): 141-50. | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/23371 | |
dc.description.abstract | Interaction of leukocyte function associated antigen-1 (LFA-1) on T-lymphoctytes and intercellular adhesion molecule-1 (ICAM-1) on epithelial cells controls leukocyte adhesion, spreading, and extravasation. This process plays an important role in leukocyte recruitment to a specific site of inflammation and has been indentified as a biomarker for certain types of carcinomas. Cyclo-(1,12)-PenITDGEATDSGC (cLABL) has been shown to inhibit LFA-1 and ICAM-1 interaction via binding to ICAM-1. In addition, cLABL has been shown to internalize after binding ICAM-1. The possibility of using cLABL conjugated nanoparticles (cLABL-NP) as a targeted and controlled release drug delivery system has been investigated in this study. The cLABL peptide was conjugated to a modified Pluronic® surfactant on poly (DL-lactic-co-glycolic acid) (PLGA) nanoparticles. The cLABL-NP showed more rapid cellular uptake by A549 lung epithelial cells compared to nanoparticles without peptide. The specificity of ICAM-1 mediated internalization was confirmed by blocking the uptake of cLABL-NP to ICAM-1 using free cLABL peptide to block the binding of cLABL-NP to ICAM-1 on the cell surface. Cell studies suggested that cLABL-NPs targeted encapsulated doxorubicin to ICAM-1 expressing cells. Cytotoxicity assay confirmed the activity of the drug incorporated in nanoparticles. Sustained release of doxorubicin afforded by PLGA nanoparticles may enable cLABL-NP as a targeted, controlled release drug delivery system. | en_US |
dc.publisher | Elsevier | en_US |
dc.rights | This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License 3.0 (CC BY-NC-ND 3.0 US), which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/ | |
dc.subject | ICAM-1 | en_US |
dc.subject | PLGA | en_US |
dc.subject | Nanoparticles | en_US |
dc.subject | Targeting | en_US |
dc.title | ICAM-1 Targeting of Doxorubicin-Loaded PLGA Nanoparticles to Lung Epithelial Cells | en_US |
dc.type | Article | en_US |
kusw.kuauthor | Berkland, Cory | |
kusw.kudepartment | Pharmaceutical Chemistry | en_US |
dc.identifier.doi | 10.1016/j.ejps.2009.02.008 | en_US |
kusw.oaversion | Scholarly/refereed, author accepted manuscript | en_US |
kusw.oapolicy | This item meets KU Open Access policy criteria. | en_US |
dc.rights.accessrights | openAccess |
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Except where otherwise noted, this item's license is described as: This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License 3.0 (CC BY-NC-ND 3.0 US), which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.