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    Microsphere-based scaffolds encapsulating chondroitin sulfate or decellularized cartilage

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    AFD_Gupta_JBiomaterAppl_2016.pdf (2.013Mb)
    Issue Date
    2016-09
    Author
    Gupta, Vineet
    Tenny, Kevin M.
    Barragan, Marilyn
    Berkland, Cory J.
    Detamore, Michael S.
    Publisher
    SAGE Publications
    Type
    Article
    Article Version
    Scholarly/refereed, publisher version
    Rights
    © The Author(s) 2016
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    Abstract
    Extracellular matrix materials such as decellularized cartilage (DCC) and chondroitin sulfate (CS) may be attractive chondrogenic materials for cartilage regeneration. The goal of the current study was to investigate the effects of encapsulation of DCC and CS in homogeneous microsphere-based scaffolds, and to test the hypothesis that encapsulation of these extracellular matrix materials would induce chondrogenesis of rat bone marrow stromal cells. Four different types of homogeneous scaffolds were fabricated from microspheres of poly(D,L-lactic-co-glycolic acid): Blank (poly(D,L-lactic-co-glycolic acid) only; negative control), transforming growth factor-β3 encapsulated (positive control), DCC encapsulated, and CS encapsulated. These scaffolds were then seeded with rat bone marrow stromal cells and cultured for 6 weeks. The DCC and CS encapsulation altered the morphological features of the microspheres, resulting in higher porosities in these groups. Moreover, the mechanical properties of the scaffolds were impacted due to differences in the degree of sintering, with the CS group exhibiting the highest compressive modulus. Biochemical evidence suggested a mitogenic effect of DCC and CS encapsulation on rat bone marrow stromal cells with the matrix synthesis boosted primarily by the inherently present extracellular matrix components. An important finding was that the cell seeded CS and DCC groups at week 6 had up to an order of magnitude higher glycosaminoglycan contents than their acellular counterparts. Gene expression results indicated a suppressive effect of DCC and CS encapsulation on rat bone marrow stromal cell chondrogenesis with differences in gene expression patterns existing between the DCC and CS groups. Overall, DCC and CS were easily included in microsphere-based scaffolds; however, there is a requirement to further refine their concentrations to achieve the differentiation profiles we seek in vitro.
    URI
    http://hdl.handle.net/1808/23242
    DOI
    https://doi.org/10.1177/0885328216655469
    Collections
    • Pharmaceutical Chemistry Scholarly Works [288]
    Citation
    Gupta, V., Tenny, K. M., Barragan, M., Berkland, C. J., & Detamore, M. S. (2016). Microsphere-based scaffolds encapsulating chondroitin sulfate or decellularized cartilage. Journal of Biomaterials Applications, 31(3), 328–343. http://doi.org/10.1177/0885328216655469

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    KU Libraries
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    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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