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    Development of an On-animal Separation-based Sensor for Monitoring Drug Metabolism in Freely Roaming Sheep

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    Issue Date
    2016-06-07
    Author
    Scott, David E.
    Willis, Sean Dustin
    Gabbert, Seth
    Johnson, David A.
    Naylor, Erik
    Janle, Elsa M.
    Krichevsky, Janice E.
    Lunte, Craig E.
    Lunte, Susan M.
    Publisher
    Royal Society of Chemistry
    Type
    Article
    Article Version
    Scholarly/refereed, author accepted manuscript
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    Abstract
    The development of an on-animal separation-based sensor that can be employed for monitoring drug metabolism in a freely roaming sheep is described. The system consists of microdialysis sampling coupled directly to microchip electrophoresis with electrochemical detection (MD-ME-EC). Separations were accomplished using an all-glass chip with integrated platinum working and reference electrodes. Discrete samples from the microdialysis flow were introduced into the electrophoresis chip using a flow-gated injection approach. Electrochemical detection was accomplished in-channel using a two-electrode isolated potentiostat. Nitrite was separated by microchip electrophoresis using reverse polarity and a run buffer consisting of 50 mM phosphate at pH 7.4. The entire system was under telemetry control. The system was first tested with rats to monitor the production of nitrite following introduction of nitroglycerin into the subdermal tissue using a linear probe. The data acquired using the on-line MD-ME-EC system was compared to that obtained off-line analysis by liquid chromatography with electrochemical detection (LC-EC), using a second microdialysis probe implanted parallel to the first probe in the same animal. The MD-ME-EC device was then used on-animal to monitor the subdermal metabolism of nitroglycerin in sheep. The ultimate goal is to use this device to simultaneously monitor drug metabolism and behavior in a freely roaming animal.
    URI
    http://hdl.handle.net/1808/22621
    DOI
    https://doi.org/10.1039/c4an01928h
    Collections
    • Chemistry Scholarly Works [557]
    Citation
    Scott, David E., Sean D. Willis, Seth Gabbert, David Johnson, Erik Naylor, Elsa M. Janle, Janice E. Krichevsky, Craig E. Lunte, and Susan M. Lunte. "Development of an On-animal Separation-based Sensor for Monitoring Drug Metabolism in Freely Roaming Sheep." The Analyst 140.11 (2015): 3820-829.

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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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