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A Simple Protein Precipitation-based Simultaneous Quantification of Lovastatin and Its Active Metabolite Lovastatin Acid in Human Plasma by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry using Polarity Switching
dc.contributor.author | Wujian, J. | |
dc.contributor.author | Kuan-wei, P. | |
dc.contributor.author | Sihyung, Y. | |
dc.contributor.author | Huijing, S. | |
dc.contributor.author | Mario, S. | |
dc.contributor.author | Wang, Zhuo | |
dc.date.accessioned | 2016-12-22T18:22:45Z | |
dc.date.available | 2016-12-22T18:22:45Z | |
dc.date.issued | 2015-03-30 | |
dc.identifier.citation | Wujian J, Kuan-wei P, Sihyung Y, Huijing S, Mario S, et al. (2015) A Simple Protein Precipitation-based Simultaneous Quantification of Lovastatin and Its Active Metabolite Lovastatin Acid in Human Plasma by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry using Polarity Switching. J Chromatogr Sep Tech 6:268. doi:10.4172/2157-7064.1000268 | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/22285 | |
dc.description.abstract | Lovastatin is an anti-cholesterol lactone drug indicated for the treatment of hyperlipidemia and to reduce the risk of coronary heart disease. It is converted to the β-hydroxy acid form (lovastatin acid) in vivo, which is the major pharmacologically active metabolite. Here, we describe the development and validation of an ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)-based method utilizing polarity switching for the simultaneous quantification of lovastatin and lovastatin acid in human plasma. Simple protein precipitation extraction and direct injection of the extracted samples without drying/reconstitution showed good recoveries of both analytes (~70%). The developed method exhibited satisfactory intra-day and inter-day accuracy and precision. The interconversion between lovastatin and lovastatin acid during sample preparation and storage was minimal (< 1.9%). The lower limits of quantification were 0.5 and 0.2 nM (or 0.2 and 0.084 ng/mL) for lovastatin and lovastatin acid, respectively, using only 50 μL of plasma during extraction. The validated method was successfully applied to analyze plasma samples obtained from a healthy human subject who enrolled in a clinical drug interaction study involving lovastatin. | en_US |
dc.publisher | OMICS International | en_US |
dc.rights | © 2015 Wujian J, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | en_US |
dc.rights.uri | https://creativecommons.org/licenses/by/3.0/ | en_US |
dc.subject | Lovastatin | en_US |
dc.subject | Lovastatin acid | en_US |
dc.subject | UPLC-MS/MS | en_US |
dc.subject | Polarity switching | en_US |
dc.subject | Protein precipitation extraction | en_US |
dc.subject | Pharmacokinetics | en_US |
dc.title | A Simple Protein Precipitation-based Simultaneous Quantification of Lovastatin and Its Active Metabolite Lovastatin Acid in Human Plasma by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry using Polarity Switching | en_US |
dc.type | Article | en_US |
kusw.kuauthor | Wang, Zhuo | |
kusw.kudepartment | Pharmaceutical Chemistry | en_US |
dc.identifier.doi | 10.4172/2157-7064.1000268 | en_US |
kusw.oaversion | Scholarly/refereed, publisher version | en_US |
kusw.oapolicy | This item meets KU Open Access policy criteria. | en_US |
dc.rights.accessrights | openAccess |
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Except where otherwise noted, this item's license is described as: © 2015 Wujian J, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.