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dc.contributor.authorKenjale, Roma
dc.contributor.authorWilson, Justin
dc.contributor.authorZenk, Sebastian F.
dc.contributor.authorSaurya, Saroj
dc.contributor.authorPicking, Wendy Lynn
dc.contributor.authorPicking, William D.
dc.contributor.authorBlocker, Ariel J.
dc.identifier.citationKenjale, R., Wilson, J., Zenk, S. F., Saurya, S., Picking, W. L., Picking, W. D., & Blocker, A. (2005). The needle component of the type III secreton of Shigella regulates the activity of the secretion apparatus. Journal of Biological Chemistry, 280(52), 42929-42937.en_US
dc.description.abstractGram-negative bacteria commonly interact with eukaryotic host cells by using type III secretion systems (TTSSs or secretons). TTSSs serve to transfer bacterial proteins into host cells. Two translocators, IpaB and IpaC, are first inserted with the aid of IpaD by Shigella into the host cell membrane. Then at least two supplementary effectors of cell invasion, IpaA and IpgD, are transferred into the host cytoplasm. How TTSSs are induced to secrete is unknown, but their activation appears to require direct contact of the external distal tip of the apparatus with the host cell. The extracellular domain of the TTSS is a hollow needle protruding 60 nm beyond the bacterial surface. The monomeric unit of the Shigella flexneri needle, MxiH, forms a superhelical assembly. To probe the role of the needle in the activation of the TTSS for secretion, we examined the structure-function relationship of MxiH by mutagenesis. Most point mutations led to normal needle assembly, but some led to polymerization or possible length control defects. In other mutants, secretion was constitutively turned “on.” In a further set, it was “constitutively on” but experimentally “uninducible.” Finally, upon induction of secretion, some mutants released only the translocators and not the effectors. Most types of mutants were defective in interactions with host cells. Together, these data indicate that the needle directly controls the activity of the TTSS and suggest that it may be used to “sense” host cells.en_US
dc.publisherAmerican Society for Biochemistry and Molecular Biologyen_US
dc.rightsThis research was originally published in Journal of Biological Chemistry. Roma Kenjale, Justin Wilson, Sebastian F. Zenk, Saroj Saurya, Wendy L. Picking, William D. Picking and Ariel Blocker. The needle component of the type III secreton of Shigella regulates the activity of the secretion apparatus. Journal of Biological Chemistry. 2005; 280, 42929-42937. © the American Society for Biochemistry and Molecular Biology.en_US
dc.titleThe Needle Component of the Type III Secreton of Shigella Regulates the Activity of the Secretion Apparatusen_US
kusw.kuauthorPicking, Wendy Lynn
kusw.kuauthorPicking, William D.
kusw.kudepartmentPharmaceutical Chemistryen_US
kusw.oanotesPer SHERPA/RoMEO 9/7/2016: Author's Pre-print: green tick author can archive pre-print (ie pre-refereeing) Author's Post-print: green tick author can archive post-print (ie final draft post-refereeing) Publisher's Version/PDF: grey tick subject to Restrictions below, author can archive publisher's version/PDF General Conditions:

Author's pre-print on not-for-profit server Author's post-print on author's personal website or institutional repository only Publisher's version/PDF may be used after 12 months embargo Must link to publisher version Set phrase to accompany deposit (See policy) Publisher automatically deposits articles in PubMed Central after 12 months embargo
kusw.oaversionScholarly/refereed, publisher versionen_US
kusw.oapolicyThis item does not meet KU Open Access policy criteria.en_US

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