dc.contributor.author | Johnson, Steven | |
dc.contributor.author | Roversi, Pietro | |
dc.contributor.author | Espina, Marianela | |
dc.contributor.author | Olive, Andrew J. | |
dc.contributor.author | Deane, Janet E. | |
dc.contributor.author | Birket, Susan | |
dc.contributor.author | Field, Terry | |
dc.contributor.author | Picking, William D. | |
dc.contributor.author | Blocker, Ariel J. | |
dc.contributor.author | Galyov, Edouard E. | |
dc.contributor.author | Picking, Wendy Lynn | |
dc.contributor.author | Lea, Susan M. | |
dc.date.accessioned | 2016-09-07T17:48:56Z | |
dc.date.available | 2016-09-07T17:48:56Z | |
dc.date.issued | 2007-02-09 | |
dc.identifier.citation | Johnson, S., Roversi, P., Espina, M., Olive, A., Deane, J. E., Birket, S., … Lea, S. M. (2007). Self-Chaperoning of the Type III Secretion System needle tip proteins IpaD and BipD. The Journal of Biological Chemistry, 282(6), 4035–4044. http://doi.org/10.1074/jbc.M607945200 | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/21474 | |
dc.description.abstract | Bacteria expressing type III secretion systems (T3SS) have been responsible for the deaths of millions worldwide, acting as key virulence elements in diseases ranging from plague to typhoid fever. The T3SS is composed of a basal body, which traverses both bacterial membranes, and an external needle through which effector proteins are secreted. We report multiple crystal structures of two proteins that sit at the tip of the needle and are essential for virulence; IpaD from Shigella flexneri and BipD from Burkholderia pseudomallei. The structures reveal that the N-terminal domains of the molecules are intra-molecular chaperones that prevent premature oligomerization, as well as sharing structural homology with proteins involved in eukaryotic actin rearrangement. Crystal packing has allowed us to construct a model for the tip complex that is supported by mutations designed using the structure. | en_US |
dc.publisher | American Society for Biochemistry and Molecular Biology | en_US |
dc.rights | This research was originally published in Journal of Biological Chemistry. Steven Johnson, Pietro Roversi, Marianela Espina, Andrew Olive, Janet E. Deane, Susan Birket, Terry Field, William D. Picking, Ariel J. Blocker, Edouard E. Galyov, Wendy L. Picking and Susan M. Lea. Self-Chaperoning of the Type III Secretion System needle tip proteins IpaD and BipD. Journal of Biological Chemistry. 2007; 282, 4035-4044. © the American Society for Biochemistry and Molecular Biology. | en_US |
dc.title | Self-chaperoning of the Type III Secretion System Needle Tip Proteins IpaD and BipD | en_US |
dc.type | Article | en_US |
kusw.kuauthor | Picking, Wendy Lynn | |
kusw.kuauthor | Picking, William D. | |
kusw.kudepartment | Pharmaceutical Chemistry | en_US |
dc.identifier.doi | 10.1074/jbc.M607945200 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0003-3441-3113
https://orcid.org/0000-0001-7998-0643 | |
kusw.oaversion | Scholarly/refereed, publisher version | en_US |
kusw.oapolicy | This item does not meet KU Open Access policy criteria. | en_US |
dc.rights.accessrights | openAccess | |