KUKU

KU ScholarWorks

  • myKU
  • Email
  • Enroll & Pay
  • KU Directory
    • Login
    View Item 
    •   KU ScholarWorks
    • Pharmaceutical Chemistry
    • Pharmaceutical Chemistry Scholarly Works
    • View Item
    •   KU ScholarWorks
    • Pharmaceutical Chemistry
    • Pharmaceutical Chemistry Scholarly Works
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Deoxycholate Interacts with IpaD of Shigella flexneri in Inducing the Recruitment of IpaB to the Type III Secretion Apparatus Needle Tip

    Thumbnail
    View/Open
    Picking_2008.pdf (425.7Kb)
    Issue Date
    2008-05-01
    Author
    Strensrud, Kenneth F.
    Adam, Philip R.
    La Mar, Cassandra D.
    Olive, Andrew J.
    Lushington, Gerald H.
    Sudharsan, Raghavi
    Shelton, Naomi L.
    Givens, Richard S.
    Picking, Wendy Lynn
    Picking, William D.
    Publisher
    American Society for Biochemistry and Molecular Biology
    Type
    Article
    Article Version
    Scholarly/refereed, publisher version
    Rights
    This research was originally published in Journal of Biological Chemistry. Kenneth F. Stensrud, Philip R. Adam, Cassandra D. La Mar, Andrew J. Olive, Gerald H. Lushington, Raghavi Sudharsan, Naomi L. Shelton, Richard S. Givens, Wendy L. Picking and William D. Picking. Deoxycholate Interacts with IpaD of Shigella flexneri in Inducing the Recruitment of IpaB to the Type III Secretion Apparatus Needle Tip. Journal of Biological Chemistry. 2008; 283, 18646-18654. © the American Society for Biochemistry and Molecular Biology.
    Metadata
    Show full item record
    Abstract
    Type III secretion (TTS) is an essential virulence function for Shigella flexneri that delivers effector proteins that are responsible for bacterial invasion of intestinal epithelial cells. The Shigella TTS apparatus (TTSA) consists of a basal body that spans the bacterial inner and outer membranes and a needle exposed at the pathogen surface. At the distal end of the needle is a “tip complex” composed of invasion plasmid antigen D (IpaD). IpaD not only regulates TTS, but is required for the recruitment and stable association of the translocator protein IpaB at the TTSA needle tip in the presence of deoxycholate or other bile salts. This phenomenon is not accompanied by induction of TTS or the recruitment of IpaC to the Shigella surface. We now show that IpaD specifically binds fluorescein-labeled deoxycholate and, based on energy transfer measurements and docking simulations, this interaction appears to occur where the N-terminal domain of IpaD meets its central coiled-coil, a region that may also be involved in needle-tip interactions. TTS is initiated as a series of distinct steps and that small molecules present in the bacterial milieu are capable of inducing the first step of TSS through interactions with the needle tip protein IpaD. Furthermore, the amino acids proposed to be important for deoxycholate binding by IpaD appear to have significant roles in regulating tip complex composition and pathogen entry into host cells.
    URI
    http://hdl.handle.net/1808/21472
    DOI
    https://doi.org/10.1074/jbc.M802799200
    Collections
    • Pharmaceutical Chemistry Scholarly Works [340]
    Citation
    Stensrud, K. F., Adam, P. R., La Mar, C. D., Olive, A. J., Lushington, G. H., Sudharsan, R., … Picking, W. D. (2008). Deoxycholate Interacts with IpaD of Shigella flexneri in Inducing the Recruitment of IpaB to the Type III Secretion Apparatus Needle Tip. The Journal of Biological Chemistry, 283(27), 18646–18654. http://doi.org/10.1074/jbc.M802799200

    Items in KU ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.


    We want to hear from you! Please share your stories about how Open Access to this item benefits YOU.


    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

    Browse

    All of KU ScholarWorksCommunities & CollectionsThis Collection

    My Account

    Login

    Statistics

    View Usage Statistics

    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

    The University of Kansas
      Contact KU ScholarWorks
    Lawrence, KS | Maps
     
    • Academics
    • Admission
    • Alumni
    • Athletics
    • Campuses
    • Giving
    • Jobs

    The University of Kansas prohibits discrimination on the basis of race, color, ethnicity, religion, sex, national origin, age, ancestry, disability, status as a veteran, sexual orientation, marital status, parental status, gender identity, gender expression and genetic information in the University’s programs and activities. The following person has been designated to handle inquiries regarding the non-discrimination policies: Director of the Office of Institutional Opportunity and Access, IOA@ku.edu, 1246 W. Campus Road, Room 153A, Lawrence, KS, 66045, (785)864-6414, 711 TTY.

     Contact KU
    Lawrence, KS | Maps